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EDD antibody, C-term

Anti-EDD antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX89818

Cat No. GTX89818

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Application

IHC-P

Reactivity

Human
Package
100 μg ($369)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
IHC-P 1-2μg/ml

Note :

IHC-P
Human Testis shows nuclear staining in all germ cells.

Not tested in other applications.

Calculated MW

309 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX89818-PEP).

Predict Reactivity

Mouse, Rat, Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-LAIKTKNFGFV, from the C Terminus of the protein sequence according to NP_056986.2.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

ubiquitin protein ligase E3 component n-recognin 5 , DD5 , EDD , EDD1 , HYD

Cellular Localization

Nucleus

Background

This gene encodes a progestin-induced protein, which belongs to the HECT (homology to E6-AP carboxyl terminus) family. The HECT family proteins function as E3 ubiquitin-protein ligases, targeting specific proteins for ubiquitin-mediated proteolysis. This gene is localized to chromosome 8q22 which is disrupted in a variety of cancers. This gene potentially has a role in regulation of cell proliferation or differentiation. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-EDD antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX89818

GTX89818 IHC-P Image

GTX89818 (4μg/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining. These results could not be obtained after antigen retrieval at pH 6.

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 369