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EDD antibody

KO/KD
Anti-EDD antibody used in Western Blot (WB). GTX00902
Anti-EDD antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00902

Cat. No. GTX00902

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse
Package
100 μg ($399)
See all EDD products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
ICC/IF 1:1000
Not tested in other applications.

Calculated MW

309 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Filter-sterilized PBS, 50% Glycerol

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide of human EDD protein corresponding to 394-408 amino acids, C-KWSESEPYRNAQNPS, conjugated with KLH

Purification

Purified by antigen-affinity chromatography
From serum

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

DD5 , EDD , EDD1 , HYD , UBR5 , ubiquitin protein ligase E3 component nrecognin 5

Cellular Localization

Nucleus

Background

This gene encodes a progestin-induced protein, which belongs to the HECT (homology to E6-AP carboxyl terminus) family. The HECT family proteins function as E3 ubiquitin-protein ligases, targeting specific proteins for ubiquitin-mediated proteolysis. This gene is localized to chromosome 8q22 which is disrupted in a variety of cancers. This gene potentially has a role in regulation of cell proliferation or differentiation. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-EDD antibody used in Western Blot (WB). GTX00902

GTX00902 WB Image

KO/KD

WB analysis of various samples using GTX00902 EDD antibody.
Lane 1 : HeLa cells transfected with control siRNA
Lane 2 : HeLa cells transfected with EDD-specific siRNA
Lane 3 : HEK293T cells transfected with pFlag-CMV-5b empty vector
Lane 4 : HEK293T cells transfected with pCMV-Tag2b-EDD expression vector

Anti-EDD antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00902

GTX00902 ICC/IF Image

ICC/IF analysis of MCF-7 cells using GTX00902 EDD antibody.
Fixation : 4% PFA, overnight
Permeabilization : 0.25% Triton X-100 in PBS, 10 min
Dilution : 1:1000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Package List Price ($)
$ 399