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EDD antibody

Anti-EDD antibody used in Western Blot (WB). GTX130759
Anti-EDD antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX130759

Cat No. GTX130759

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

309 kDa. ( Note )

Positive Control

U87-MG , U87-MG nuclear extract

Predict Reactivity

Mouse, Zebrafish, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

3.04 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human EDD. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886340

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

ubiquitin protein ligase E3 component n-recognin 5 , DD5 , EDD , EDD1 , HYD

Cellular Localization

Nucleus

Background

This gene encodes a progestin-induced protein, which belongs to the HECT (homology to E6-AP carboxyl terminus) family. The HECT family proteins function as E3 ubiquitin-protein ligases, targeting specific proteins for ubiquitin-mediated proteolysis. This gene is localized to chromosome 8q22 which is disrupted in a variety of cancers. This gene potentially has a role in regulation of cell proliferation or differentiation. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-EDD antibody used in Western Blot (WB). GTX130759

GTX130759 WB Image

U87-MG whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with EDD antibody (GTX130759) diluted at 1:500.

Anti-EDD antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX130759

GTX130759 ICC/IF Image

EDD antibody detects EDD protein at nucleus by immunofluorescent analysis.
Sample: U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: EDD protein stained by EDD antibody (GTX130759) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169