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EDG1 antibody [C2C3], C-term

Anti-EDG1 antibody [C2C3], C-term used in Western Blot (WB). GTX100024
Anti-EDG1 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100024
Anti-EDG1 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100024
Anti-EDG1 antibody [C2C3], C-term used in Western Blot (WB). GTX100024
Anti-EDG1 antibody [C2C3], C-term used in Western Blot (WB). GTX100024

Cat. No. GTX100024

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all EDG1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

43 kDa. ( Note )

Positive Control

HepG2

Predict Reactivity

Rat, Rabbit, Bovine, Dog, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide encompassing a sequence within the Intracellular domain of human EDG1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240738

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

sphingosine-1-phosphate receptor 1 , CD363 , CHEDG1 , D1S3362 , ECGF1 , EDG-1 , EDG1 , S1P1

Cellular Localization

Cell membrane; Multi-pass membrane protein

Background

The protein encoded by this gene is structurally similar to G protein-coupled receptors and is highly expressed in endothelial cells. It binds the ligand sphingosine-1-phosphate with high affinity and high specificity, and suggested to be involved in the processes that regulate the differentiation of endothelial cells. Activation of this receptor induces cell-cell adhesion. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-EDG1 antibody [C2C3], C-term used in Western Blot (WB). GTX100024

GTX100024 WB Image

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with EDG1 antibody [C2C3], C-term (GTX100024) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-EDG1 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100024

GTX100024 ICC/IF Image

EDG1 antibody [C2C3], C-term detects EDG1 protein at cytoplasm by immunofluorescent analysis.
Sample: HCT116 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: EDG1 protein stained by EDG1 antibody [C2C3], C-term (GTX100024) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-EDG1 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100024

GTX100024 IHC-P Image

Immunohistochemical analysis of paraffin-embedded mouse brain, using EDG1(GTX100024) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-EDG1 antibody [C2C3], C-term used in Western Blot (WB). GTX100024

GTX100024 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with EDG1 antibody [C2C3], C-term (GTX100024) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-EDG1 antibody [C2C3], C-term used in Western Blot (WB). GTX100024

GTX100024 WB Image

Sample(30 μg of whole cell lysate)
A:Hep G2 (GTX27900)
12% SDS PAGE
GTX100024 diluted at 1:1000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Document
Unmodified.pdf
Package List Price ($)
$ 399
$ 169