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EDG1 antibody [C2C3], C-term

Anti-EDG1 antibody [C2C3], C-term used in Western Blot (WB). GTX100024
Anti-EDG1 antibody [C2C3], C-term used in Western Blot (WB). GTX100024
Anti-EDG1 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100024
Anti-EDG1 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100024

Cat No. GTX100024

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

43 kDa. ( Note )

Positive Control

HepG2 , mouse liver
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human EDG1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

sphingosine-1-phosphate receptor 1,CD363,CHEDG1,D1S3362,ECGF1,EDG-1,EDG1,S1P1

Cellular Localization

Cell membrane; Multi-pass membrane protein

Background

The protein encoded by this gene is structurally similar to G protein-coupled receptors and is highly expressed in endothelial cells. It binds the ligand sphingosine-1-phosphate with high affinity and high specificity, and suggested to be involved in the processes that regulate the differentiation of endothelial cells. Activation of this receptor induces cell-cell adhesion. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-EDG1 antibody [C2C3], C-term used in Western Blot (WB). GTX100024

GTX100024 WB Image

Sample(30 μg of whole cell lysate)
A:Hep G2 (GTX27900)
12% SDS PAGE
GTX100024 diluted at 1:1000

Anti-EDG1 antibody [C2C3], C-term used in Western Blot (WB). GTX100024

GTX100024 WB Image

EDG1 antibody [C2C3], C-term detects S1PR1 protein by Western blot analysis.
A. 50 µg mouse liver lysate/extract
10 % SDS-PAGE
EDG1 antibody [C2C3], C-term (GTX100024) dilution: 1:1000

Anti-EDG1 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100024

GTX100024 ICC/IF Image

EDG1 antibody [C2C3], C-term detects EDG1 protein at cytoplasm by immunofluorescent analysis.
Sample: HCT116 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: EDG1 protein stained by EDG1 antibody [C2C3], C-term (GTX100024) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-EDG1 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100024

GTX100024 IHC-P Image

Immunohistochemical analysis of paraffin-embedded mouse brain, using EDG1(GTX100024) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
There are currently no references for EDG1 antibody [C2C3], C-term (GTX100024). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for EDG1 antibody [C2C3], C-term (GTX100024). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Document
Unmodified.pdf
Package List Price ($)
$ 319
$ 169