GeneTex
United States (US)

EDG1 antibody [C2C3], C-term

GTX100024_WB_19011505_626.jpg
GTX100024_39471_WB_M_liver_19011506_893.jpg
GTX100024_39471_20160113_IFA_19011506_342.jpg
GTX100024_39471_IHC_19011506_560.jpg

Cat No. GTX100024

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

43 kDa. ( Note )

Positive Control

HepG2 , mouse liver

Predict Reactivity

Rat, Zebrafish, Rabbit, Bovine, Xenopus laevis, Xenopus tropicalis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human EDG1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CHEDG1 antibody, D1S3362 antibody, ECGF1 antibody, EDG-1 antibody, EDG1 antibody, FLJ58121 antibody, S1P1 antibody, S1PR1 antibody, sphingosine 1-phosphate receptor Edg-1 antibody, endothelial differentiation, sphingolipid G-protein-coupled receptor, 1 antibody, S1P receptor 1 antibody, endothelial differentiation G-protein coupled receptor 1 antibody, S1P receptor Edg-1 antibody, sphingosine 1-phosphate receptor EDG1 antibody, sphingosine 1-phosphate receptor 1 antibody, sphingosine-1-phosphate receptor 1 antibody

Cellular Localization

Cell membrane; Multi-pass membrane protein

Background

The protein encoded by this gene is structurally similar to G protein-coupled receptors and is highly expressed in endothelial cells. It binds the ligand sphingosine-1-phosphate with high affinity and high specificity, and suggested to be involved in the processes that regulate the differentiation of endothelial cells. Activation of this receptor induces cell-cell adhesion. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX100024_WB_19011505_626.jpg

GTX100024 WB Image

Sample(30 μg of whole cell lysate)
A:Hep G2 (GTX27900)
12% SDS PAGE
GTX100024 diluted at 1:1000

GTX100024_39471_WB_M_liver_19011506_893.jpg

GTX100024 WB Image

EDG1 antibody [C2C3], C-term detects S1PR1 protein by Western blot analysis.
A. 50 µg mouse liver lysate/extract
10 % SDS-PAGE
EDG1 antibody [C2C3], C-term (GTX100024) dilution: 1:1000

GTX100024_39471_20160113_IFA_19011506_342.jpg

GTX100024 ICC/IF Image

EDG1 antibody [C2C3], C-term detects EDG1 protein at cytoplasm by immunofluorescent analysis.
Sample: HCT116 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: EDG1 protein stained by EDG1 antibody [C2C3], C-term (GTX100024) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

GTX100024_39471_IHC_19011506_560.jpg

GTX100024 IHC-P Image

Immunohistochemical analysis of paraffin-embedded mouse brain, using EDG1(GTX100024) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Document
Unmodified.pdf
Package List Price ($)
$ 319
$ 169