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EDG1 antibody

Anti-EDG1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX11424
Anti-EDG1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX11424
Anti-EDG1 antibody used in Western Blot (WB). GTX11424
Anti-EDG1 antibody used in Western Blot (WB). GTX11424

Cat. No. GTX11424

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-Fr, FACS, IP, IHC

Reactivity

Human, Mouse, Rat
Package
50 μg ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 2-4 μg/ml
ICC/IF 2 μg/ml
IHC-Fr Assay dependent
FACS 3-5 μg/10⁶ cells
IP Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

43 kDa. ( Note )

Product Note

This antibody shows no cross-reactivity to S1P3. Immunofluorescence data demonstrates that S1P1 is localized to the plasma membrane.

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Synthetic peptide corresponding to residues S(359) H P Q K D D G D N P E T I(372) of mouse S1P1.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

sphingosine-1-phosphate receptor 1 , CD363 , CHEDG1 , D1S3362 , ECGF1 , EDG-1 , EDG1 , S1P1

Cellular Localization

Cell membrane; Multi-pass membrane protein,Endosome

Background

The protein encoded by this gene is structurally similar to G protein-coupled receptors and is highly expressed in endothelial cells. It binds the ligand sphingosine-1-phosphate with high affinity and high specificity, and suggested to be involved in the processes that regulate the differentiation of endothelial cells. Activation of this receptor induces cell-cell adhesion. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016]

Database

Research Area

DATA IMAGES

Anti-EDG1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX11424

GTX11424 ICC/IF Image

ICC/IF analysis of S1P1 transfected CCL39 cells using GTX11424 EDG1 antibody.

Anti-EDG1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX11424

GTX11424 ICC/IF Image

ICC/IF analysis of Neuro-2a cells using GTX11424 EDG1 antibody. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Trito X-100 for 10 minutes
Dilution : 2 μg/ml in 0.1% BSA and incubated for 3 hours at room temperature

Anti-EDG1 antibody used in Western Blot (WB). GTX11424

GTX11424 WB Image

WB analysis of rat Brain (Lane 1) and Neuro- 2a (Lane 2) lysate using GTX11424 EDG1 antibody.
Dilution : 2 μg/ml

Anti-EDG1 antibody used in Western Blot (WB). GTX11424

GTX11424 WB Image

WB analysis of transfected HEK293 cell lysate using GTX11424 EDG1 antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399