APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1 - 2 μg/mL |
| 2.5 μg/mL |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
Mouse Thymus Tissue Lysate(GTX24028) , Rat Spleen Tissue Lysate(GTX27945)
Product Note
At least two isoforms of S1P1 are known to exist; this S1P1 antibody will only recognize the shorter isoform.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
EDG1 antibody was raised against a 14 amino acid synthetic peptide near the carboxy terminus of the human EDG1.The immunogen is located within the last 50 amino acids of EDG1.
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
sphingosine-1-phosphate receptor 1 , CD363 , CHEDG1 , D1S3362 , ECGF1 , EDG-1 , EDG1 , S1P1
Cellular Localization
Cell membrane; Multi-pass membrane protein,Endosome
Background
The protein encoded by this gene is structurally similar to G protein-coupled receptors and is highly expressed in endothelial cells. It binds the ligand sphingosine-1-phosphate with high affinity and high specificity, and suggested to be involved in the processes that regulate the differentiation of endothelial cells. Activation of this receptor induces cell-cell adhesion. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX31360 WB Image
WB analysis of mouse thymus tissue lysate using GTX31360 EDG1 antibody.
Working concentration : (A) 1 and (B) 2 μg/ml
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GTX31360 IHC-P Image
IHC-P analysis of mouse thymus tissue using GTX31360 EDG1 antibody.
Working concentration : 5 μg/ml
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GTX31360 IHC-P Image
IHC-P analysis of mouse thymus tissue using GTX31360 EDG1 antibody.
Working concentration : 2.5 μg/ml
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GTX31360 IHC-P Image
IHC-P analysis of mouse thymus tissue using GTX31360 EDG1 antibody.
Green : Primary antibody
Blue : DAPI
Dilution : 20 μg/ml
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REFERENCE
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REVIEW
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