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EDTA buffer, 10X pH 8.0

Cat No. GTX30937

Application

IHC-P
Package
100 ml ($89)
PRODUCT

Summary

EDTA Antigen Retrieval Buffer, 10X pH 8.0

APPLICATION

Application Note

Deparafinize FFPE tissue section is carried out as outlined in your protocol. Hydrate tissue with PBS. The tissue slides are immersed in this 1X solution and heated for 10-20 minutes at 100ºC; OR in pressure cooker for 3-5 minutes followed by cooling at room temperature for about 20 minutes. The tissue sections are ready for further IHC protocol.

PROPERTIES

Form

Liquid

Storage

2-8ºC, DO NOT FREEZE.

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Background

This buffer is intended for heat-induced antigen retriever on formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. In IHC most commonly used fixative like formalin mask tissue antigens (cellular, membrane and nuclear) by cross-linking process, this results in poor or no staining in IHC. The use of this buffer on FFPE tissue section improves accessibility of antibodies to tissue antigens.

REFERENCE

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REVIEW

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Package List Price ($)
$ 89