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EEA1 antibody [C3], C-term

Anti-EEA1 antibody [C3], C-term used in Western Blot (WB). GTX109638
Anti-EEA1 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX109638
Anti-EEA1 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109638
Anti-EEA1 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109638
Anti-EEA1 antibody [C3], C-term used in Western Blot (WB). GTX109638
Anti-EEA1 antibody [C3], C-term used in Western Blot (WB). GTX109638
Anti-EEA1 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX109638
Anti-EEA1 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX109638
Anti-EEA1 antibody [C3], C-term used in Western Blot (WB). GTX109638

Cat No. GTX109638

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat, Hamster
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

162 kDa. ( Note )

Positive Control

HepG2 , A375 , mouse brain , PC-12 , Rat-2 , *A549 , *MNT-1

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human EEA1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

early endosome antigen 1 , MST105 , MSTP105 , ZFYVE2

Cellular Localization

Cytoplasm , Early endosome membrane

Background

Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in endosomal trafficking.

Database

Research Area

DATA IMAGES

Anti-EEA1 antibody [C3], C-term used in Western Blot (WB). GTX109638

GTX109638 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membranes were blotted with EEA1 antibody [C3], C-term (GTX109638) diluted at 1:5000 and competitor's antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-EEA1 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX109638

GTX109638 IHC-P Image

EEA1 antibody [C3], C-term detects EEA1 protein at cytosol on mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
EEA1 antibody [C3], C-term (GTX109638) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-EEA1 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109638

GTX109638 ICC/IF Image

EEA1 antibody [C3], C-term detects EEA1 protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: EEA1 protein stained by EEA1 antibody [C3], C-term (GTX109638) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT886] (GTX631830) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-EEA1 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109638

GTX109638 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using EEA1(GTX109638) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Anti-EEA1 antibody [C3], C-term used in Western Blot (WB). GTX109638

GTX109638 WB Image

EEA1 antibody [C3], C-term detects EEA1 protein by western blot analysis.
A. 30 μg HepG2 whole cell lysate/extract
B. 30 μg A375 whole cell lysate/extract
5% SDS-PAGE
EEA1 antibody [C3], C-term (GTX109638) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-EEA1 antibody [C3], C-term used in Western Blot (WB). GTX109638

GTX109638 WB Image

Sample (50 μg of whole cell lysate)
A: Mouse brain
5% SDS PAGE
GTX109638 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-EEA1 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX109638

GTX109638 IHC-P Image

EEA1 antibody [C3], C-term detects EEA1 protein at cytosol on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded colon carcinoma.
EEA1 antibody [C3], C-term (GTX109638) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-EEA1 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX109638

GTX109638 IHC-P Image

EEA1 antibody [C3], C-term detects EEA1 protein at cytosol on mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
EEA1 antibody [C3], C-term (GTX109638) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-EEA1 antibody [C3], C-term used in Western Blot (WB). GTX109638

GTX109638 WB Image

EEA1 antibody [C3], C-term detects EEA1 protein by western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
B. 30 μg Rat2 whole cell lysate/extract
5% SDS-PAGE
EEA1 antibody [C3], C-term (GTX109638) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

EEA1 antibody [C3], C-term Cat No. GTX109638

Rating ( Average 4 based on 2 users reviews)
Application
Western Blot(WB) ( Average 4 based on 2 users reviews)
Date : Anonymous submitted on 24-Apr-2015
Score :
Application Tested : WB
Sample Species : Hu
Sample : MNT-1 cells
Amount used : 30μg
Blocking : 5% skim milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Date : Anonymous submitted on 25-Oct-2013
Score :
Application Tested : WB
Sample Species : Hu
Sample : A549 cells
Amount used : 40μg
Blocking : 5% not-fat milk, 25°C, 2Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169