GeneTex
  • Country / Location Selection

United States (US)

EEA1 antibody [C3], C-term

Anti-EEA1 antibody [C3], C-term used in Western Blot (WB). GTX109638
Anti-EEA1 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX109638
Anti-EEA1 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109638
Anti-EEA1 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109638
Anti-EEA1 antibody [C3], C-term used in Western Blot (WB). GTX109638
Anti-EEA1 antibody [C3], C-term used in Western Blot (WB). GTX109638
Anti-EEA1 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX109638
Anti-EEA1 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX109638
Anti-EEA1 antibody [C3], C-term used in Western Blot (WB). GTX109638

Cat No. GTX109638

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat, Hamster
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

162 kDa. ( Note )

Positive Control

HepG2 , A375 , mouse brain , PC-12 , Rat-2 , *A549 , *MNT-1

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human EEA1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950162

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

early endosome antigen 1 , MST105 , MSTP105 , ZFYVE2

Cellular Localization

Cytoplasm , Early endosome membrane

Background

Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in endosomal trafficking.

Database

Research Area

DATA IMAGES

Anti-EEA1 antibody [C3], C-term used in Western Blot (WB). GTX109638

GTX109638 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membranes were blotted with EEA1 antibody [C3], C-term (GTX109638) diluted at 1:5000 and competitor's antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-EEA1 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX109638

GTX109638 IHC-P Image

EEA1 antibody [C3], C-term detects EEA1 protein at cytosol on mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
EEA1 antibody [C3], C-term (GTX109638) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-EEA1 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109638

GTX109638 ICC/IF Image

EEA1 antibody [C3], C-term detects EEA1 protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: EEA1 protein stained by EEA1 antibody [C3], C-term (GTX109638) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT886] (GTX631830) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-EEA1 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109638

GTX109638 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using EEA1(GTX109638) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Anti-EEA1 antibody [C3], C-term used in Western Blot (WB). GTX109638

GTX109638 WB Image

EEA1 antibody [C3], C-term detects EEA1 protein by western blot analysis.
A. 30 μg HepG2 whole cell lysate/extract
B. 30 μg A375 whole cell lysate/extract
5% SDS-PAGE
EEA1 antibody [C3], C-term (GTX109638) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-EEA1 antibody [C3], C-term used in Western Blot (WB). GTX109638

GTX109638 WB Image

Sample (50 μg of whole cell lysate)
A: Mouse brain
5% SDS PAGE
GTX109638 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-EEA1 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX109638

GTX109638 IHC-P Image

EEA1 antibody [C3], C-term detects EEA1 protein at cytosol on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded colon carcinoma.
EEA1 antibody [C3], C-term (GTX109638) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-EEA1 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX109638

GTX109638 IHC-P Image

EEA1 antibody [C3], C-term detects EEA1 protein at cytosol on mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
EEA1 antibody [C3], C-term (GTX109638) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-EEA1 antibody [C3], C-term used in Western Blot (WB). GTX109638

GTX109638 WB Image

EEA1 antibody [C3], C-term detects EEA1 protein by western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
B. 30 μg Rat2 whole cell lysate/extract
5% SDS-PAGE
EEA1 antibody [C3], C-term (GTX109638) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

EEA1 antibody [C3], C-term Cat No. GTX109638

Rating ( Average 4.6 based on 5 users reviews)
Application
Western Blot(WB) ( Average 4.3 based on 3 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 5 based on 2 users reviews)
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2016.PMID: 26838546
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : ICC/IF
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Virol J in 2012.PMID: 22339884
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : ICC/IF
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Virol J in 2012.PMID: 22339884
Date : Anonymous submitted on 24-Apr-2015
Score :
Application Tested : WB
Sample Species : Hu
Sample : MNT-1 cells
Amount used : 30μg
Blocking : 5% skim milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Date : Anonymous submitted on 25-Oct-2013
Score :
Application Tested : WB
Sample Species : Hu
Sample : A549 cells
Amount used : 40μg
Blocking : 5% not-fat milk, 25°C, 2Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169