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EEA1 antibody

Anti-EEA1 antibody used in Western Blot (WB). GTX22900
Anti-EEA1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22900
Anti-EEA1 antibody used in Western Blot (WB). GTX22900

Cat. No. GTX22900

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, FACS, IHC

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all EEA1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 2 μg/mL
ICC/IF 2 μg/mL
FACS 3-5 μg/10⁶ cells
IHC Assay dependent
Not tested in other applications.

Calculated MW

161 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide corresponding to residues T(1391) P S S K K P V R V C D A C F N D L Q G(1410) of human EEA1.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

early endosome antigen 1 , A430109M19Rik , B230358H09Rik , ZFYVE2

Cellular Localization

Cytoplasm

Background

Early endosomal antigen 1 (EEA1) is a 162 kDa membrane bound protein component specific to the early endosome and is essential for fusion between early endocytic vesicles. Early endosomes are cytoplasmic compartments which fuse with endocytic vesicles for redistribution of extracellular compounds to alternate destinations. Zinc-finger-like domains, reminiscent of those found in nucleic acid binding proteins, are located in the amino and carboxyl-terminal domains of EEA1. The carboxyl-terminal zinc-finger-like-domain is conserved in several other non-nuclear proteins, some of which are also involved in intracellular protein trafficking. In addition, this domain is an authentic zinc-binding domain and is critical to the intracellular localization of EEA1. Membrane association of EEA1 has been shown dependent on phosphatidyl 3-kinase activity, inhibitors of which cause EEA1 to dissociate from early endosomes.

Database

Research Area

DATA IMAGES

Anti-EEA1 antibody used in Western Blot (WB). GTX22900

GTX22900 WB Image

WB analysis of HeLa (Lane 1), Hep G2 (Lane 2) A-431 (Lane 3) PC-12 (Lane 4), SH-SY5Y (Lane 5), NIH/3T3 (Lane 6), Mouse brain tissue (Lane 7) and Rat brain tissue (Lane 8) using GTX22900 EEA1 antibody.
Dilution : 2 μg/ml

Anti-EEA1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22900

GTX22900 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX22900 EEA1 antibody. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 for 10 minute
Dilution : 2 μg/ml in 0.1% BSA and incubated for 3 hours at room temperature

Anti-EEA1 antibody used in Western Blot (WB). GTX22900

GTX22900 WB Image

WB analysis of mouse brain using GTX22900 EEA1 antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399