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EED antibody [GT885]

Anti-EED antibody [GT885] used in Western Blot (WB). GTX634650
Anti-EED antibody [GT885] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX634650
Anti-EED antibody [GT885] used in IHC (Paraffin sections) (IHC-P). GTX634650
Anti-EED antibody [GT885] used in IHC (Paraffin sections) (IHC-P). GTX634650

Cat. No. GTX634650

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT885

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all EED products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Positive Control

A549 , H1299 , HCT116

Predict Reactivity

Zebrafish, Bovine, Chicken, Japanese Medaka, Xenopus laevis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.54 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human EED. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888470

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

embryonic ectoderm development , COGIS , HEED , WAIT1

Cellular Localization

Nucleus

Background

This gene encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein interacts with enhancer of zeste 2, the cytoplasmic tail of integrin beta7, immunodeficiency virus type 1 (HIV-1) MA protein, and histone deacetylase proteins. This protein mediates repression of gene activity through histone deacetylation, and may act as a specific regulator of integrin function. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-EED antibody [GT885] used in Western Blot (WB). GTX634650

GTX634650 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with EED antibody [GT885] (GTX634650) diluted at 1:500. The HRP-conjugated anti-mouset IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-EED antibody [GT885] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX634650

GTX634650 ICC/IF Image

EED antibody [GT885] detects EED protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: EED stained by EED antibody [GT885] (GTX634650) diluted at 1:1000.
Blue: Hoechst 33342 staining.

Anti-EED antibody [GT885] used in IHC (Paraffin sections) (IHC-P). GTX634650

GTX634650 IHC-P Image

EED antibody [GT885] detects EED protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat endometrial carcinoma.
EED stained by EED antibody [GT885] (GTX634650) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-EED antibody [GT885] used in IHC (Paraffin sections) (IHC-P). GTX634650

GTX634650 IHC-P Image

EED antibody [GT885] detects EED protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
EED stained by EED antibody [GT885] (GTX634650) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations