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EEF1A2 antibody [C1C3]

Anti-EEF1A2 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX102327
Anti-EEF1A2 antibody [C1C3] used in Western Blot (WB). GTX102327
Anti-EEF1A2 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX102327
Anti-EEF1A2 antibody [C1C3] used in Western Blot (WB). GTX102327
Anti-EEF1A2 antibody [C1C3] used in Western Blot (WB). GTX102327

Cat No. GTX102327

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji , mouse brain , rat brain

Specificity/Sensitivity

Based on a BLAST result, it may also cross with human EEF1A1.

Predict Reactivity

Zebrafish, Rabbit, Bovine, Chicken, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human EEF1A2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

eukaryotic translation elongation factor 1 alpha 2 , EEF1AL , EF-1-alpha-2 , EF1A , EIEE33 , HS1 , MRD38 , STN , STNL

Cellular Localization

Nucleus

Background

This gene encodes an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 2) is expressed in brain, heart and skeletal muscle, and the other isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas. This gene may be critical in the development of ovarian cancer. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-EEF1A2 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX102327

GTX102327 IHC-P Image

EEF1A2 antibody [C1C3] detects EEF1A2 protein at cytosol on mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
EEF1A2 antibody [C1C3] (GTX102327) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-EEF1A2 antibody [C1C3] used in Western Blot (WB). GTX102327

GTX102327 WB Image

Rat tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with EEF1A2 antibody [C1C3] (GTX102327) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-EEF1A2 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX102327

GTX102327 IHC-P Image

EEF1A2 antibody [C1C3] detects EEF1A2 protein at cytosol on rat fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat fore brain.
EEF1A2 antibody [C1C3] (GTX102327) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-EEF1A2 antibody [C1C3] used in Western Blot (WB). GTX102327

GTX102327 WB Image

Sample (50 µg of whole cell lysate)
A: mouse brain
10% SDS PAGE
GTX102327 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-EEF1A2 antibody [C1C3] used in Western Blot (WB). GTX102327

GTX102327 WB Image

Sample(30 µg whole cell lysate)
A: 293T
B: A431 (GTX27909)
C: H1299
D: HeLa S3 (GTX14654)
E: HepG2 (GTX27900)
F: MOLT4 (GTX27912)
G: Raji (GTX27908)
10% SDS PAGE
GTX102327 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
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REVIEW
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169