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EEF1A2 antibody [C1C3]

Anti-EEF1A2 antibody [C1C3] used in Western Blot (WB). GTX102327
Anti-EEF1A2 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX102327
Anti-EEF1A2 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX102327
Anti-EEF1A2 antibody [C1C3] used in Western Blot (WB). GTX102327
Anti-EEF1A2 antibody [C1C3] used in Western Blot (WB). GTX102327

Cat. No. GTX102327

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all EEF1A2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Positive Control

Mouse brain , 293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji , rat brain

Product Note

Based on a BLAST result, it may also cross with human EEF1A1.

Predict Reactivity

Zebrafish, Rabbit, Bovine, Chicken, Japanese Medaka, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.66 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human EEF1A2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950165

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

eukaryotic translation elongation factor 1 alpha 2 , EEF1AL , EF-1-alpha-2 , EF1A , EIEE33 , HS1 , MRD38 , STN , STNL

Cellular Localization

Nucleus

Background

This gene encodes an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 2) is expressed in brain, heart and skeletal muscle, and the other isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas. This gene may be critical in the development of ovarian cancer. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-EEF1A2 antibody [C1C3] used in Western Blot (WB). GTX102327

GTX102327 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with EEF1A2 antibody [C1C3] (GTX102327) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-EEF1A2 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX102327

GTX102327 IHC-P Image

EEF1A2 antibody [C1C3] detects EEF1A2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissue.
EEF1A2 stained by EEF1A2 antibody [C1C3] (GTX102327) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-EEF1A2 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX102327

GTX102327 IHC-P Image

EEF1A2 antibody [C1C3] detects EEF1A2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat tissue.
EEF1A2 stained by EEF1A2 antibody [C1C3] (GTX102327) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-EEF1A2 antibody [C1C3] used in Western Blot (WB). GTX102327

GTX102327 WB Image

Sample (50 μg of whole cell lysate)
A: mouse brain
10% SDS PAGE
GTX102327 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-EEF1A2 antibody [C1C3] used in Western Blot (WB). GTX102327

GTX102327 WB Image

Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with EEF1A2 antibody [C1C3] (GTX102327) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169