GeneTex
United States (US)

EEF1A2 antibody

KO/KD
GTX102326_40100_20181221_WB_shRNA_watermark_19011519_273.jpg
GTX102326_42705_20170329_IHC-P_R_19011506_752.jpg
GTX102326_40100_20160421_WB_R_brain_19011506_424.jpg
GTX102326_40100_20161222_WB_19011506_199.jpg
GTX102326_40100_WB_M_muscle_19011506_940.jpg
GTX102326_40100_WB_M_brain_19011506_285.jpg
GTX102326_42705_20170329_IHC-P_M_19011506_527.jpg

Cat No. GTX102326

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , Mouse brain , mouse muscle , rat brain , EEF1A2 shRNA-transfected 293T

Specificity/Sensitivity

Based on a BLAST result, it may also cross with human EEF1A1.

Predict Reactivity

Zebrafish, Rabbit, Bovine, Chicken, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Concentration

0.85mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human EEF1A2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

EEF1AL antibody, EF-1-alpha-2 antibody, EF1A antibody, FLJ41696 antibody, HS1 antibody, STN antibody, STNL antibody, EEF1A2 antibody, statin-S1 antibody, elongation factor 1-alpha 2 antibody, elongation factor-1 alpha antibody, statin S1 antibody, eukaryotic elongation factor 1 A-2 antibody, eEF1A-2 antibody, eukaryotic translation elongation factor 1 alpha 2 antibody

Cellular Localization

Nucleus

Background

This gene encodes an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 2) is expressed in brain, heart and skeletal muscle, and the other isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas. This gene may be critical in the development of ovarian cancer. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
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Database

Research Area

DATA IMAGES
GTX102326_40100_20181221_WB_shRNA_watermark_19011519_273.jpg

GTX102326 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with EEF1A2 antibody (GTX102326) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX102326_42705_20170329_IHC-P_R_19011506_752.jpg

GTX102326 IHC-P Image

EEF1A2 antibody detects EEF1A2 protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
EEF1A2 antibody (GTX102326) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX102326_40100_20160421_WB_R_brain_19011506_424.jpg

GTX102326 WB Image

Rat tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with EEF1A2 antibody (GTX102326) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX102326_40100_20161222_WB_19011506_199.jpg

GTX102326 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with EEF1A2 antibody (GTX102326) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX102326_40100_WB_M_muscle_19011506_940.jpg

GTX102326 WB Image

EEF1A2 antibody detects EEF1A2 protein by western blot analysis.
A. 50 µg mouse muscle lysate/extract
10% SDS-PAGE
EEF1A2 antibody (GTX102326) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX102326_40100_WB_M_brain_19011506_285.jpg

GTX102326 WB Image

Sample (50 µg of whole cell lysate)
A: Mouse brain
10% SDS PAGE
GTX102326 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX102326_42705_20170329_IHC-P_M_19011506_527.jpg

GTX102326 IHC-P Image

EEF1A2 antibody detects EEF1A2 protein at cytoplasm in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
EEF1A2 antibody (GTX102326) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Reference
SDS
Glycerol.pdf
Thimerosal.pdf