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EEF1A2 antibody

KO/KD
Anti-EEF1A2 antibody used in Western Blot (WB). GTX102326
Anti-EEF1A2 antibody used in Western Blot (WB). GTX102326
Anti-EEF1A2 antibody used in IHC (Paraffin sections) (IHC-P). GTX102326
Anti-EEF1A2 antibody used in IHC (Paraffin sections) (IHC-P). GTX102326
Anti-EEF1A2 antibody used in Western Blot (WB). GTX102326
Anti-EEF1A2 antibody used in Western Blot (WB). GTX102326
Anti-EEF1A2 antibody used in Western Blot (WB). GTX102326
Anti-EEF1A2 antibody used in Western Blot (WB). GTX102326
Anti-EEF1A2 antibody used in IHC (Paraffin sections) (IHC-P). GTX102326

Cat. No. GTX102326

Reference 8

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all EEF1A2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Positive Control

Mouse brain , mouse muscle , rat brain , 293T

Product Note

Based on a BLAST result, it may also cross with human EEF1A1.

Predict Reactivity

Rabbit, Bovine, Chicken, Japanese Medaka, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.21 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human EEF1A2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950166

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

eukaryotic translation elongation factor 1 alpha 2 , EEF1AL , EF-1-alpha-2 , EF1A , EIEE33 , HS1 , MRD38 , STN , STNL

Cellular Localization

Nucleus

Background

This gene encodes an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 2) is expressed in brain, heart and skeletal muscle, and the other isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas. This gene may be critical in the development of ovarian cancer. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-EEF1A2 antibody used in Western Blot (WB). GTX102326

GTX102326 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with EEF1A2 antibody (GTX102326) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-EEF1A2 antibody used in Western Blot (WB). GTX102326

GTX102326 WB Image

Various tissue extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with EEF1A2 antibody (GTX102326) diluted at 1:1000.

Anti-EEF1A2 antibody used in IHC (Paraffin sections) (IHC-P). GTX102326

GTX102326 IHC-P Image

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using EEF1A2 antibody (GTX102326) at 1:300 dilution.

Anti-EEF1A2 antibody used in IHC (Paraffin sections) (IHC-P). GTX102326

GTX102326 IHC-P Image

EEF1A2 antibody detects EEF1A2 protein at cytoplasm in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
EEF1A2 antibody (GTX102326) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-EEF1A2 antibody used in Western Blot (WB). GTX102326

GTX102326 WB Image

Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with EEF1A2 antibody (GTX102326) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-EEF1A2 antibody used in Western Blot (WB). GTX102326

GTX102326 WB Image

Sample (50 μg of whole cell lysate)
A: Mouse brain
10% SDS PAGE
GTX102326 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-EEF1A2 antibody used in Western Blot (WB). GTX102326

GTX102326 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with EEF1A2 antibody (GTX102326) diluted at 1:1000.

Anti-EEF1A2 antibody used in Western Blot (WB). GTX102326

GTX102326 WB Image

EEF1A2 antibody detects EEF1A2 protein by western blot analysis.
A. 50 μg mouse muscle lysate/extract
10% SDS-PAGE
EEF1A2 antibody (GTX102326) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-EEF1A2 antibody used in IHC (Paraffin sections) (IHC-P). GTX102326

GTX102326 IHC-P Image

EEF1A2 antibody detects EEF1A2 protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
EEF1A2 antibody (GTX102326) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

M Yamato et al. Pathol Int 2024; High expression of eukaryotic elongation factor 1?alpha?2 in lung adenocarcinoma is associated with poor prognosis
Xu Y et al. J Biol Chem 2023; 299 (6) : 104802 Lactate promotes neuronal differentiation of SH-SY5Y cells by lactate-responsive gene sets through NDRG3-dependent and -independent manners.
Hassan MK et al. Life Sci 2020; 262 : 118553 EEF1A2 triggers stronger ERK mediated metastatic program in ER negative breast cancer cells than in ER positive cells.
Application : WB
Reactivity : Human
Hassan MK et al. Life Sci 2020; 246 : 117399 Expression pattern of EEF1A2 in brain tumors: Histological analysis and functional role as a promoter of EMT.
Application : WB
Reactivity : Human
Losada A et al. Br J Cancer 2018; 119 (11) : 1410-1420 Binding of eEF1A2 to the RNA-dependent protein kinase PKR modulates its activity and promotes tumour cell survival.
Application : WB
Reactivity : Human
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REVIEW

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Application Reference
M Yamato et al. Pathol Int 2024; High expression of eukaryotic elongation factor 1?alpha?2 in lung adenocarcinoma is associated with poor prognosis
Xu Y et al. J Biol Chem 2023; 299 (6):104802 Lactate promotes neuronal differentiation of SH-SY5Y cells by lactate-responsive gene sets through NDRG3-dependent and -independent manners.
Hassan MK et al. Life Sci 2020; 262:118553 EEF1A2 triggers stronger ERK mediated metastatic program in ER negative breast cancer cells than in ER positive cells.
Application : WB
Reactivity : Human
Hassan MK et al. Life Sci 2020; 246:117399 Expression pattern of EEF1A2 in brain tumors: Histological analysis and functional role as a promoter of EMT.
Application : WB
Reactivity : Human
Losada A et al. Br J Cancer 2018; 119 (11):1410-1420 Binding of eEF1A2 to the RNA-dependent protein kinase PKR modulates its activity and promotes tumour cell survival.
Application : WB
Reactivity : Human
Barbe C et al. J Proteome Res 2017; (Epub) Comparative Proteomic and Transcriptomic Analysis of Follistatin-induced Skeletal Muscle Hypertrophy.
Application : WB
Reactivity : Mouse
Losada A et al. Sci Rep 2016; 6:35100 Translation Elongation Factor eEF1A2 is a Novel Anticancer Target for the Marine Natural Product Plitidepsin.
Application : WB
Reactivity : Human
Doig J et al. FEBS J 2013; 280 (24):6528-40 In vivo characterization of the role of tissue-specific translation elongation factor 1A2 in protein synthesis reveals insights into muscle atrophy.
Application : WB
Reactivity : Mouse
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169