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EEF1B2 antibody [N1C3]

Anti-EEF1B2 antibody [N1C3] used in Western Blot (WB). GTX106535
Anti-EEF1B2 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX106535
Anti-EEF1B2 antibody [N1C3] used in Western Blot (WB). GTX106535
Anti-EEF1B2 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106535
Anti-EEF1B2 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX106535

Cat. No. GTX106535

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

Positive Control

Mouse brain , A431 , H1299 , HeLaS3

Predict Reactivity

Rat, Zebrafish, Rabbit, Bovine, Chicken, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human EEF1B2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950167

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

eukaryotic translation elongation factor 1 beta 2 , EEF1B , EEF1B1 , EF1B

Background

This gene encodes a translation elongation factor. The protein is a guanine nucleotide exchange factor involved in the transfer of aminoacylated tRNAs to the ribosome. Alternative splicing results in three transcript variants which differ only in the 5' UTR. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-EEF1B2 antibody [N1C3] used in Western Blot (WB). GTX106535

GTX106535 WB Image

Sample(30 ug whole cell lysate)
A:A431(GTX27909)
B:H1299
C:HeLa S3(GTX14654)
12% SDS PAGE
GTX106535 diluted at 1:1000

Anti-EEF1B2 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX106535

GTX106535 IHC-P Image

Immunohistochemical analysis of paraffin-embedded U87 xenograft, using EEF1B2(GTX106535) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-EEF1B2 antibody [N1C3] used in Western Blot (WB). GTX106535

GTX106535 WB Image

Sample (50 ug of whole cell lysate)
A: mouse brain
12% SDS PAGE
GTX106535 diluted at 1:5000

Anti-EEF1B2 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106535

GTX106535 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using EF-1-beta2(GTX106535) antibody at 1:200 dilution.

Anti-EEF1B2 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX106535

GTX106535 IHC-P Image

EEF1B2 antibody [N1C3] detects EEF1B2 protein at cytoplasm on mouse duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
EEF1B2 antibody [N1C3] (GTX106535) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

There are currently no references for EEF1B2 antibody [N1C3] (GTX106535). Be the first to share your publications with this product.

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169