GeneTex
United States (US)

EEF1B2 antibody [N1C3]

GTX106535_39959_20150402_IHC_M_19011506_110.jpg
GTX106535_39959_IHC_19011506_222.jpg
GTX106535_WB_19011506_830.jpg
GTX106535_39959_WB_M_brain_19011506_781.jpg
GTX106535_IFA_19011506_866.jpg

Cat No. GTX106535

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji , mouse brain

Predict Reactivity

Rat, Zebrafish, Rabbit, Bovine, Chicken, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.)

Concentration

1mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human EEF1B2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

EEF1B antibody, EEF1B1 antibody, EF1B antibody, EEF1B2 antibody, elongation factor 1-beta antibody, eukaryotic translation elongation factor 1 beta 1 antibody, EF-1-beta antibody, eukaryotic translation elongation factor 1 beta 2 antibody

Background

This gene encodes a translation elongation factor. The protein is a guanine nucleotide exchange factor involved in the transfer of aminoacylated tRNAs to the ribosome. Alternative splicing results in three transcript variants which differ only in the 5' UTR. [provided by RefSeq]
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Database

DATA IMAGES
GTX106535_39959_20150402_IHC_M_19011506_110.jpg

GTX106535 IHC-P Image

EEF1B2 antibody [N1C3] detects EEF1B2 protein at cytoplasm on mouse duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
EEF1B2 antibody [N1C3] (GTX106535) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX106535_39959_IHC_19011506_222.jpg

GTX106535 IHC-P Image

Immunohistochemical analysis of paraffin-embedded U87 xenograft, using EEF1B2(GTX106535) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX106535_WB_19011506_830.jpg

GTX106535 WB Image

Sample(30 ug whole cell lysate)
A:A431(GTX27909)
B:H1299
C:HeLa S3(GTX14654)
12% SDS PAGE
GTX106535 diluted at 1:1000

GTX106535_39959_WB_M_brain_19011506_781.jpg

GTX106535 WB Image

Sample (50 ug of whole cell lysate)
A: mouse brain
12% SDS PAGE
GTX106535 diluted at 1:5000

GTX106535_IFA_19011506_866.jpg

GTX106535 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using EF-1-beta2(GTX106535) antibody at 1:200 dilution.

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169