GeneTex
United States (US)

EEF1D antibody [N1C3]

GTX102292_39911_IFA_19011506_614.jpg
GTX102292_WB_19011506_592.jpg
GTX102292_39911_WB_M_brain_19011506_448.jpg

Cat No. GTX102292

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

31 kDa. ( Note )

Positive Control

A431 , H1299 , HeLa , HepG2 , mouse brain

Predict Reactivity

Rat, Sheep, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.91 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human EEF1D. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Eukaryotic Translation Elongation Factor 1 Delta,Ef-1D,Ef1D,Fp1047,Eef1D

Background

This gene encodes a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This subunit functions as guanine nucleotide exchange factor. It is reported that this subunit interacts with HIV-1 Tat, and thus it represses the translation of host-cell, but not HIV-1, mRNAs. Several alternatively spliced transcript variants encoding multiple isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX102292_39911_IFA_19011506_614.jpg

GTX102292 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using EEF1D(GTX102292) antibody at 1:200 dilution.

GTX102292_WB_19011506_592.jpg

GTX102292 WB Image

Sample (30 ug of whole cell lysate)
A: H1299
12% SDS PAGE
GTX102292 diluted at 1:1000

GTX102292_39911_WB_M_brain_19011506_448.jpg

GTX102292 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
10% SDS PAGE
GTX102292 diluted at 1:3000

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169