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EEF1D antibody [N1C3]

Anti-EEF1D antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102292
Anti-EEF1D antibody [N1C3] used in Western Blot (WB). GTX102292
Anti-EEF1D antibody [N1C3] used in Western Blot (WB). GTX102292

Cat. No. GTX102292

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all EEF1D products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

31 kDa. ( Note )

Positive Control

H1299 , Mouse brain

Predict Reactivity

Rat, Sheep, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.91 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human EEF1D. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036825

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

eukaryotic translation elongation factor 1 delta , EF-1D , EF1D , FP1047

Background

This gene encodes a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This subunit functions as guanine nucleotide exchange factor. It is reported that this subunit interacts with HIV-1 Tat, and thus it represses the translation of host-cell, but not HIV-1, mRNAs. Several alternatively spliced transcript variants encoding multiple isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-EEF1D antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102292

GTX102292 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using EEF1D(GTX102292) antibody at 1:200 dilution.

Anti-EEF1D antibody [N1C3] used in Western Blot (WB). GTX102292

GTX102292 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
10% SDS PAGE
GTX102292 diluted at 1:3000

Anti-EEF1D antibody [N1C3] used in Western Blot (WB). GTX102292

GTX102292 WB Image

Sample (30 ug of whole cell lysate)
A: H1299
12% SDS PAGE
GTX102292 diluted at 1:1000

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169