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EEF1D antibody

Anti-EEF1D antibody used in IHC (Paraffin sections) (IHC-P). GTX111151
Anti-EEF1D antibody used in Western Blot (WB). GTX111151
Anti-EEF1D antibody used in Western Blot (WB). GTX111151
Anti-EEF1D antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111151

Cat. No. GTX111151

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all EEF1D products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

31 kDa. ( Note )

Positive Control

A549 , THP-1 , HL-60 , NCI-H929

Predict Reactivity

Mouse, Rat, Sheep, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human EEF1D. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11162753

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

eukaryotic translation elongation factor 1 delta , EF-1D , EF1D , FP1047

Background

This gene encodes a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This subunit functions as guanine nucleotide exchange factor. It is reported that this subunit interacts with HIV-1 Tat, and thus it represses the translation of host-cell, but not HIV-1, mRNAs. Several alternatively spliced transcript variants encoding multiple isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-EEF1D antibody used in IHC (Paraffin sections) (IHC-P). GTX111151

GTX111151 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human adenocarcinoma, using EEF1D(GTX111151) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-EEF1D antibody used in Western Blot (WB). GTX111151

GTX111151 WB Image

Sample (30 ug of whole cell lysate)
A: A549
10% SDS PAGE
GTX111151 diluted at 1:1000

Anti-EEF1D antibody used in Western Blot (WB). GTX111151

GTX111151 WB Image

EEF1D antibody detects EEF1D protein by Western blot analysis.
A. 30 μg THP-1 whole cell lysate/extract
B. 30 μg HL-60 whole cell lysate/extract
C. 30 μg NCI-H929 whole cell lysate/extract
10 % SDS-PAGE
EEF1D antibody (GTX111151) dilution: 1:1000

Anti-EEF1D antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111151

GTX111151 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using EEF1D(GTX111151) antibody at 1:500 dilution.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169