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EEF1E1 antibody [N1C3]

KO/KD
Anti-EEF1E1 antibody [N1C3] used in Western Blot (WB). GTX105658
Anti-EEF1E1 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105658
Anti-EEF1E1 antibody [N1C3] used in Western Blot (WB). GTX105658
Anti-EEF1E1 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX105658
Anti-EEF1E1 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX105658
Anti-EEF1E1 antibody [N1C3] used in Western Blot (WB). GTX105658

Cat. No. GTX105658

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all EEF1E1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

20 kDa. ( Note )

Positive Control

Mouse brain , A431 , 293T

Predict Reactivity

Rat, Bovine, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human EEF1E1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950168

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

eukaryotic translation elongation factor 1 epsilon 1 , AIMP3 , P18

Cellular Localization

Cytoplasm , Nucleus

Background

This gene encodes a multifunctional protein that localizes both in the cytoplasm and in the nucleus. In the cytoplasm, the encoded protein is an auxiliary component of the macromolecular aminoacyl-tRNA synthase complex. However, its mouse homolog has been shown to translocate to the nucleus in response to DNA damage and plays a positive role in ATM/ATR-mediated p53 activation. Alternative splicing results in multiple transcript variants. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-EEF1E1 antibody [N1C3] used in Western Blot (WB). GTX105658

GTX105658 WB Image

KO/KD

Wild-type (WT) and EEF1E1 knockout (KO) 293T cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with EEF1E1 antibody [N1C3] (GTX105658) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-EEF1E1 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105658

GTX105658 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using EEF1E1(GTX105658) antibody at 1:200 dilution.

Anti-EEF1E1 antibody [N1C3] used in Western Blot (WB). GTX105658

GTX105658 WB Image

Sample(30 μg of whole cell lysate)
A:A431(GTX27909)
12% SDS PAGE
GTX105658 diluted at 1:1000

Anti-EEF1E1 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX105658

GTX105658 IHC-P Image

EEF1E1 antibody [N1C3] detects EEF1E1 protein at cytoplasm on mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
EEF1E1 antibody [N1C3] (GTX105658) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-EEF1E1 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX105658

GTX105658 IHC-P Image

EEF1E1 antibody [N1C3] detects EEF1E1 protein at cytoplasm on mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
EEF1E1 antibody [N1C3] (GTX105658) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-EEF1E1 antibody [N1C3] used in Western Blot (WB). GTX105658

GTX105658 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
12% SDS PAGE
GTX105658 diluted at 1:1000

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169