GeneTex
United States (US)

EEF1E1 antibody [N1C3]

KO/KD
GTX105658_39806_20180202_WB_KO_watermark_19011506_446.jpg
GTX105658_39806_WB_M_brain_19011506_779.jpg
GTX105658_39806_20141205_IHC_M_2_19011506_632.jpg
GTX105658_WB_19011506_370.jpg
GTX105658_IFA_19011506_700.jpg
GTX105658_39806_20141205_IHC_M_19011506_839.jpg

Cat No. GTX105658

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

20 kDa. ( Note )

Positive Control

293T , A431 , Raji , mouse brain

Predict Reactivity

Rat, Bovine, Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human EEF1E1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

AIMP3 antibody, P18 antibody, EEF1E1 antibody, aminoacyl tRNA synthetase complex-interacting multifunctional protein 3 antibody, ARS-interacting multifunctional protein 3 antibody, p18 component of aminoacyl-tRNA synthetase complex antibody, multisynthase complex auxiliary component p18 antibody, eukaryotic translation elongation factor 1 epsilon-1 antibody, eukaryotic translation elongation factor 1 epsilon 1 antibody

Cellular Localization

Cytoplasm , Nucleus

Background

This gene encodes a multifunctional protein that localizes both in the cytoplasm and in the nucleus. In the cytoplasm, the encoded protein is an auxiliary component of the macromolecular aminoacyl-tRNA synthase complex. However, its mouse homolog has been shown to translocate to the nucleus in response to DNA damage and plays a positive role in ATM/ATR-mediated p53 activation. Alternative splicing results in multiple transcript variants. [provided by RefSeq]
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Database

DATA IMAGES
GTX105658_39806_20180202_WB_KO_watermark_19011506_446.jpg

GTX105658 WB Image

KO/KD

Wild-type (WT) and EEF1E1 knockout (KO) 293T cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with EEF1E1 antibody [N1C3] (GTX105658) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
CRISPR knockout cell lines generated by www.edigene.com

GTX105658_39806_WB_M_brain_19011506_779.jpg

GTX105658 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
12% SDS PAGE
GTX105658 diluted at 1:1000

GTX105658_39806_20141205_IHC_M_2_19011506_632.jpg

GTX105658 IHC-P Image

EEF1E1 antibody [N1C3] detects EEF1E1 protein at cytoplasm on mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
EEF1E1 antibody [N1C3] (GTX105658) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX105658_WB_19011506_370.jpg

GTX105658 WB Image

Sample(30 μg of whole cell lysate)
A:A431(GTX27909)
12% SDS PAGE
GTX105658 diluted at 1:1000

GTX105658_IFA_19011506_700.jpg

GTX105658 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using EEF1E1(GTX105658) antibody at 1:200 dilution.

GTX105658_39806_20141205_IHC_M_19011506_839.jpg

GTX105658 IHC-P Image

EEF1E1 antibody [N1C3] detects EEF1E1 protein at cytoplasm on mouse pancreas by immunohistochemical analysis.
Sample: Paraffin-embedded mouse pancreas.
EEF1E1 antibody [N1C3] (GTX105658) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169