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EEF1E1 antibody

Anti-EEF1E1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX66367
Anti-EEF1E1 antibody used in Western Blot (WB). GTX66367

Cat. No. GTX66367

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all EEF1E1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:200 - 1:2000
ICC/IF 1:50 - 1:200
Not tested in other applications.

Calculated MW

20 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human EEF1E1 (NP_004271.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

eukaryotic translation elongation factor 1 epsilon 1 , AIMP3 , P18

Cellular Localization

Cytoplasm,Cytoplasm, cytosol,Nucleus

Background

This gene encodes a multifunctional protein that localizes to both the cytoplasm and nucleus. In the cytoplasm, the encoded protein is an auxiliary component of the macromolecular aminoacyl-tRNA synthase complex. However, its mouse homolog has been shown to translocate to the nucleus in response to DNA damage, and it plays a positive role in ATM/ATR-mediated p53 activation. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the neighboring downstream MUTED (muted homolog) gene. An EEF1E1-related pseudogene has been identified on chromosome 2. [provided by RefSeq, Dec 2010]

Database

Research Area

DATA IMAGES

Anti-EEF1E1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX66367

GTX66367 ICC/IF Image

ICC/IF analysis of L929 cells using GTX66367 EEF1E1 antibody.
Blue : DAPI
Dilution : 1:100

Anti-EEF1E1 antibody used in Western Blot (WB). GTX66367

GTX66367 WB Image

WB analysis of various sample lysates using GTX66367 EEF1E1 antibody.
Dilution : 1:1000
Loading : 25μg per lane

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399