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EEF1G antibody [GT1278]

Recombinant antibody
Anti-EEF1G antibody [GT1278] used in Western Blot (WB). GTX03190
Anti-EEF1G antibody [GT1278] used in Western Blot (WB). GTX03190
Anti-EEF1G antibody [GT1278] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX03190
Anti-EEF1G antibody [GT1278] used in IHC (Paraffin sections) (IHC-P). GTX03190
Anti-EEF1G antibody [GT1278] used in Western Blot (WB). GTX03190
Anti-EEF1G antibody [GT1278] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX03190
Anti-EEF1G antibody [GT1278] used in IHC (Paraffin sections) (IHC-P). GTX03190
Anti-EEF1G antibody [GT1278] used in IHC (Paraffin sections) (IHC-P). GTX03190

Cat. No. GTX03190

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 GT1278

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($439)
See all EEF1G products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.32 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human EEF1G.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

eukaryotic translation elongation factor 1 gamma , EF1G , GIG35

Background

This gene encodes a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This subunit contains an N-terminal glutathione transferase domain, which may be involved in regulating the assembly of multisubunit complexes containing this elongation factor and aminoacyl-tRNA synthetases. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-EEF1G antibody [GT1278] used in Western Blot (WB). GTX03190

GTX03190 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with EEF1G antibody [GT1278] (GTX03190) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-EEF1G antibody [GT1278] used in Western Blot (WB). GTX03190

GTX03190 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with EEF1G antibody [GT1278] (GTX03190) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-EEF1G antibody [GT1278] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX03190

GTX03190 ICC/IF Image

ICC/IF analysis of NIH-3T3 cells using GTX03190 EEF1G antibody [GT1278].
Blue : DAPI for nuclear staining
Dilution : 1:100

Anti-EEF1G antibody [GT1278] used in IHC (Paraffin sections) (IHC-P). GTX03190

GTX03190 IHC-P Image

IHC-P analysis of rat brain tissue section using GTX03190 EEF1G antibody [GT1278].
Dilution : 1:100

Anti-EEF1G antibody [GT1278] used in Western Blot (WB). GTX03190

GTX03190 WB Image

WB analysis of various samples using GTX03190 EEF1G antibody [GT1278].
Dilution : 1:1000
Loading : 25μg per lane

Anti-EEF1G antibody [GT1278] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX03190

GTX03190 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX03190 EEF1G antibody [GT1278].
Blue : DAPI for nuclear staining
Dilution : 1:100

Anti-EEF1G antibody [GT1278] used in IHC (Paraffin sections) (IHC-P). GTX03190

GTX03190 IHC-P Image

IHC-P analysis of mouse brain tissue section using GTX03190 EEF1G antibody [GT1278].
Dilution : 1:100

Anti-EEF1G antibody [GT1278] used in IHC (Paraffin sections) (IHC-P). GTX03190

GTX03190 IHC-P Image

IHC-P analysis of human placenta tissue section using GTX03190 EEF1G antibody [GT1278].
Dilution : 1:100

REFERENCE

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REVIEW

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Package List Price ($)
$ 439