GeneTex

  • Country / Location Selection

United States (US)

EFEMP1 antibody [HL2572]

Recombinant antibody Orthogonal Overexpression
Anti-EFEMP1 antibody [HL2572] used in Western Blot (WB). GTX638950
Anti-EFEMP1 antibody [HL2572] used in IHC (Paraffin sections) (IHC-P). GTX638950
Anti-EFEMP1 antibody [HL2572] used in Western Blot (WB). GTX638950

Cat. No. GTX638950

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2572

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all EFEMP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human EFEMP1

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

EGF containing fibulin extracellular matrix protein 1 , DHRD , DRAD , FBLN3 , FBNL , FIBL-3 , MLVT , MTLV , S1-5

Cellular Localization

Secreted, extracellular space, extracellular matrix

Background

This gene encodes a member of the fibulin family of extracellular matrix glycoproteins. Like all members of this family, the encoded protein contains tandemly repeated epidermal growth factor-like repeats followed by a C-terminus fibulin-type domain. This gene is upregulated in malignant gliomas and may play a role in the aggressive nature of these tumors. Mutations in this gene are associated with Doyne honeycomb retinal dystrophy. Alternatively spliced transcript variants that encode the same protein have been described.[provided by RefSeq, Nov 2009]

Database

Research Area

DATA IMAGES

Anti-EFEMP1 antibody [HL2572] used in Western Blot (WB). GTX638950

GTX638950 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with EFEMP1 antibody [HL2572] (GTX638950) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-EFEMP1 antibody [HL2572] used in IHC (Paraffin sections) (IHC-P). GTX638950

GTX638950 IHC-P Image

EFEMP1 antibody [HL2572] detects secreted EFEMP1 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse stomach.
EFEMP1 stained by EFEMP1 antibody [HL2572] (GTX638950) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-EFEMP1 antibody [HL2572] used in Western Blot (WB). GTX638950

GTX638950 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with EFEMP1 antibody [HL2572] (GTX638950) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

REFERENCE

There are currently no references for EFEMP1 antibody [HL2572] (GTX638950). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for EFEMP1 antibody [HL2572] (GTX638950). Be the first to share your experience with this product.
Submit a Review
Package List Price ($)
$ 399
$ 169
Custom Conjugations