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EFEMP1 antibody

Orthogonal Overexpression
Anti-EFEMP1 antibody used in Western Blot (WB). GTX111656
Anti-EFEMP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX111656
Anti-EFEMP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111656
Anti-EFEMP1 antibody used in Western Blot (WB). GTX111656

Cat. No. GTX111656

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all EFEMP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

Positive Control

Raji

Predict Reactivity

Mouse, Rat, Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human EFEMP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10720785

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

EGF containing fibulin extracellular matrix protein 1 , DHRD , DRAD , FBLN3 , FBNL , FIBL-3 , MLVT , MTLV , S1-5

Cellular Localization

Secreted

Background

This gene spans approximately 18 kb of genomic DNA and consists of 12 exons. Alternative splice patterns in the 5' UTR result in three transcript variants encoding the same extracellular matrix protein. Mutations in this gene are associated with Doyne honeycomb retinal dystrophy. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-EFEMP1 antibody used in Western Blot (WB). GTX111656

GTX111656 WB Image

Orthogonal

Various whole cell extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with EFEMP1 antibody (GTX111656) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-EFEMP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX111656

GTX111656 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using EFEMP1(GTX111656) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-EFEMP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111656

GTX111656 ICC/IF Image

EFEMP1 antibody detects EFEMP1 protein at cytoplasm by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: EFEMP1 protein stained by EFEMP1 antibody (GTX111656) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-EFEMP1 antibody used in Western Blot (WB). GTX111656

GTX111656 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with EFEMP1 antibody (GTX111656) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169