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EFEMP1 antibody

Anti-EFEMP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX14926
Anti-EFEMP1 antibody used in Western Blot (WB). GTX14926

Cat. No. GTX14926

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IP, ELISA

Reactivity

 Human, Mouse, Rat, Monkey
Package
100 μg ($399)
See all EFEMP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500
IHC-P Assay dependent
IP 1:200
ELISA 1:10000
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Tris/Glycine, 0.5% BSA, 30% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.65 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic cyclic peptide corresponding to N terminal amino acids 25-42 of Human EFEMP1, peptide was covalently modified. Sequence:TYTQCTDGYEWDPVRQQC

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_369994

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

EGF containing fibulin extracellular matrix protein 1 , DHRD , DRAD , FBLN3 , FBNL , FIBL-3 , MLVT , MTLV , S1-5

Background

This gene encodes a member of the fibulin family of extracellular matrix glycoproteins. Like all members of this family, the encoded protein contains tandemly repeated epidermal growth factor-like repeats followed by a C-terminus fibulin-type domain. This gene is upregulated in malignant gliomas and may play a role in the aggressive nature of these tumors. Mutations in this gene are associated with Doyne honeycomb retinal dystrophy. Alternatively spliced transcript variants that encode the same protein have been described.[provided by RefSeq, Nov 2009]

Database

Research Area

DATA IMAGES

Anti-EFEMP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX14926

GTX14926 IHC-P Image

IHC-P analysis of mouse eye tissue using GTX14926 EFEMP1 antibody.
Dilution : 1:50

Anti-EFEMP1 antibody used in Western Blot (WB). GTX14926

GTX14926 WB Image

WB analysis of human RPE cell lysate using GTX14926 EFEMP1 antibody.
Dilution : 1:500

REFERENCE

Application Reference

Pernemalm M et al. Proteomics 2009; 9 (13) : 3414-24 Use of narrow-range peptide IEF to improve detection of lung adenocarcinoma markers in plasma and pleural effusion.
Application : WB
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REVIEW

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Package List Price ($)
$ 399