GeneTex
United States (US)

EFEMP1 antibody

Anti-EFEMP1 antibody used in Western Blot (WB). GTX31792
Anti-EFEMP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31792
Anti-EFEMP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31792

Cat No. GTX31792

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, ELISA
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.5 - 1 μg/mL
ICC/IF 20 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

Positive Control

HeLa Cell Slide(GTX25529) , HeLa Cell Lysate

Specificity/Sensitivity

At least three isoforms of Fibulin 3 are known to exist. This antibody is predicted to not cross-react with other Fibulin proteins.
PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Fibulin 3 antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human Fibulin 3.The immunogen is located within amino acids 410 - 460 of Fibulin 3.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Egf Containing Fibulin Extracellular Matrix Protein 1,Dhrd,Drad,Fbln3,Fbnl,Fibl-3,Mlvt,Mtlv,S1-5,Efemp1

Background

This gene encodes a member of the fibulin family of extracellular matrix glycoproteins. Like all members of this family, the encoded protein contains tandemly repeated epidermal growth factor-like repeats followed by a C-terminus fibulin-type domain. This gene is upregulated in malignant gliomas and may play a role in the aggressive nature of these tumors. Mutations in this gene are associated with Doyne honeycomb retinal dystrophy. Alternatively spliced transcript variants that encode the same protein have been described.[provided by RefSeq, Nov 2009]

Database

Research Area

DATA IMAGES
Anti-EFEMP1 antibody used in Western Blot (WB). GTX31792

GTX31792 WB Image

WB analysis of HeLa cell lysate using GTX31792 EFEMP1 antibody.
Working concentration : (A) 0.5 and (B) 1 μg/ml

Anti-EFEMP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31792

GTX31792 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX31792 EFEMP1 antibody.
Working concentration : 20 μg/ml

Anti-EFEMP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31792

GTX31792 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX31792 EFEMP1 antibody.
Working concentration : 20 μg/ml

Package List Price ($)
$ 329