APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.5 - 1 μg/mL |
| 20 μg/mL |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
HeLa Cell Slide(GTX25529) , HeLa Cell Lysate
Product Note
At least three isoforms of Fibulin 3 are known to exist. This antibody is predicted to not cross-react with other Fibulin proteins.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Fibulin 3 antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human Fibulin 3.The immunogen is located within amino acids 410 - 460 of Fibulin 3.
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
EGF containing fibulin extracellular matrix protein 1 , DHRD , DRAD , FBLN3 , FBNL , FIBL-3 , MLVT , MTLV , S1-5
Background
This gene encodes a member of the fibulin family of extracellular matrix glycoproteins. Like all members of this family, the encoded protein contains tandemly repeated epidermal growth factor-like repeats followed by a C-terminus fibulin-type domain. This gene is upregulated in malignant gliomas and may play a role in the aggressive nature of these tumors. Mutations in this gene are associated with Doyne honeycomb retinal dystrophy. Alternatively spliced transcript variants that encode the same protein have been described.[provided by RefSeq, Nov 2009]
Database
Research Area
DATA IMAGES
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GTX31792 ICC/IF Image
ICC/IF analysis of HeLa cells using GTX31792 EFEMP1 antibody.
Working concentration : 20 μg/ml
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GTX31792 WB Image
WB analysis of HeLa cell lysate using GTX31792 EFEMP1 antibody.
Working concentration : (A) 0.5 and (B) 1 μg/ml
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GTX31792 ICC/IF Image
ICC/IF analysis of HeLa cells using GTX31792 EFEMP1 antibody.
Working concentration : 20 μg/ml
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REFERENCE
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REVIEW
There are currently no reviews for EFEMP1 antibody (GTX31792). Be the first to share your experience with this product.
