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EFEMP2 antibody, C-term

Anti-EFEMP2 antibody, C-term used in Flow cytometry (FACS). GTX80452
Anti-EFEMP2 antibody, C-term used in Western Blot (WB). GTX80452
Anti-EFEMP2 antibody, C-term used in Western Blot (WB). GTX80452

Cat. No. GTX80452

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, FACS

Reactivity

 Human, Mouse
Package
400 μl ($399)
See all EFEMP2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
FACS 1:10-1:50
Not tested in other applications.

Calculated MW

49 kDa. ( Note )

Predict Reactivity

Hamster(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH conjugated synthetic peptide between 386-414 amino acids from the C-terminal region of human EFEMP2.

Purification

Protein A purified, followed by peptide affinity purification.

Conjugation

Unconjugated

RRID

AB_11174383

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

EGF containing fibulin extracellular matrix protein 2 , ARCL1B , FBLN4 , MBP1 , UPH1

Cellular Localization

Secreted

Background

A large number of extracellular matrix proteins have been found to contain variations of the epidermal growth factor (EGF) domain and have been implicated in functions as diverse as blood coagulation, activation of complement and determination of cell fate during development. The protein encoded by this gene contains four EGF2 domains and six calcium-binding EGF2 domains. This gene is necessary for elastic fiber formation and connective tissue development. Defects in this gene are cause of an autosomal recessive cutis laxa syndrome. Alternatively spliced transcript variants have been identified for this gene. [provided by RefSeq, Jan 2011]

Database

Research Area

DATA IMAGES

Anti-EFEMP2 antibody, C-term used in Flow cytometry (FACS). GTX80452

GTX80452 FACS Image

FACS analysis of HepG2 cells using GTX80452 EFEMP2 antibody, C-term.
Top histogram : negative control
Bottom histogram : HepG2 cells

Anti-EFEMP2 antibody, C-term used in Western Blot (WB). GTX80452

GTX80452 WB Image

WB analysis of NIH-3T3 cell lysate (35ug/lane) using GTX80452 EFEMP2 antibody, C-term.

Anti-EFEMP2 antibody, C-term used in Western Blot (WB). GTX80452

GTX80452 WB Image

WB analysis of HepG2 cell lysate (35ug/lane) using GTX80452 EFEMP2 antibody, C-term.

REFERENCE

Application Reference

Erin N et al. Breast Cancer Res Treat 2018; (Epub) Secretomes reveal several novel proteins as well as TGF-β1 as the top upstream regulator of metastatic process in breast cancer.
Application : WB
Reactivity : Mouse
Submit a Reference

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399