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EFHD2 antibody

Overexpression
Anti-EFHD2 antibody used in IHC (Frozen sections) (IHC-Fr). GTX108080
Anti-EFHD2 antibody used in Western Blot (WB). GTX108080
Anti-EFHD2 antibody used in Western Blot (WB). GTX108080
Anti-EFHD2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108080
Anti-EFHD2 antibody used in Western Blot (WB). GTX108080

Cat. No. GTX108080

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-Fr

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all EFHD2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

27 kDa. ( Note )

Positive Control

H1299 , mouse brain , rat brain , EFHD2-transfected 293T

Predict Reactivity

Zebrafish, Bovine, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human EFHD2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950173

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

EF-hand domain family member D2 , SWS1

Background

May regulate B-cell receptor (BCR)-induced immature and primary B-cell apoptosis (By similarity). Plays a role as negative regulator of the canonical NF-kappa-B-activating branch (By similarity). Controls spontaneous apoptosis through the regulation of BCL2L1 abundance.

Database

Research Area

DATA IMAGES

Anti-EFHD2 antibody used in IHC (Frozen sections) (IHC-Fr). GTX108080

GTX108080 IHC-Fr Image

EFHD2 antibody detects EFHD2 protein expression by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: EFHD2 protein stained by EFHD2 antibody (GTX108080) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-EFHD2 antibody used in Western Blot (WB). GTX108080

GTX108080 WB Image

Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with EFHD2 antibody (GTX108080) diluted at 1:1000.

Anti-EFHD2 antibody used in Western Blot (WB). GTX108080

GTX108080 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with EFHD2 antibody (GTX108080) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-EFHD2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108080

GTX108080 ICC/IF Image

EFHD2 antibody detects EFHD2 protein by immunofluorescent analysis.
Sample: DIV14 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: EFHD2 protein stained by EFHD2 antibody (GTX108080) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-EFHD2 antibody used in Western Blot (WB). GTX108080

GTX108080 WB Image

Sample(30 μg of whole cell lysate)
A:H1299
10% SDS PAGE
GTX108080 diluted at 1:1000

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169