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EGF antibody [4E11]

Anti-EGF antibody [4E11] used in Western Blot (WB). GTX82999
Anti-EGF antibody [4E11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX82999

Cat. No. GTX82999

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 4E11

Isotype

 IgG1

Application

 WB, ICC/IF, ELISA

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all EGF products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
ICC/IF 1/200 - 1/1000
ELISA 1/10000
Not tested in other applications.

Calculated MW

134 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.03% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant fragment of human EGF expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

epidermal growth factor , HOMG4 , URG

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

This gene encodes a member of the epidermal growth factor superfamily. The encoded protein is synthesized as a large precursor molecule that is proteolytically cleaved to generate the 53-amino acid epidermal growth factor peptide. This protein acts a potent mitogenic factor that plays an important role in the growth, proliferation and differentiation of numerous cell types. This protein acts by binding the high affinity cell surface receptor, epidermal growth factor receptor. Defects in this gene are the cause of hypomagnesemia type 4. Dysregulation of this gene has been associated with the growth and progression of certain cancers. Alternate splicing results in multiple transcript variants.[provided by RefSeq, May 2010]

Database

Research Area

DATA IMAGES

Anti-EGF antibody [4E11] used in Western Blot (WB). GTX82999

GTX82999 WB Image

WB analysis of EGF(AA: 971-1023)-hIgGFc transfected HEK293 cell lysate using GTX82999 EGF antibody [4E11].

Anti-EGF antibody [4E11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX82999

GTX82999 ICC/IF Image

ICC/IF analysis of NIH3T3 cells using GTX82999 EGF antibody [4E11].
Green : EGF
Blue: DRAQ5 fluorescent DNA dye
Red: Actin filaments

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399