GeneTex
United States (US)

EGF antibody (Biotin)

Cat No. GTX12440

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, ELISA
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
IHC-P Assay dependent
ELISA Assay dependent

Note :

Rabbit anti human EGF may be used as in Western Blotting applications under either reducing or non-reducing conditions with recombinant human EGF as the positive control.
This biotinylated human EGF antibody may be used in a direct ELISA or as the detection reagent in a sandwich ELISA.
Not tested in other applications.

Calculated MW

134 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.)

Concentration

0.1mg/ml

Antigen Species

Human

Immunogen

Recombinant human EGF

Purification

Purified by affinity chromatography

Conjugation

Biotin

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

HOMG4 Antibody , URG Antibody , epidermal growth factor Antibody

Background

This gene encodes a member of the epidermal growth factor superfamily. The encoded protein is synthesized as a large precursor molecule that is proteolytically cleaved to generate the 53-amino acid epidermal growth factor peptide. This protein acts a potent mitogenic factor that plays an important role in the growth, proliferation and differentiation of numerous cell types. This protein acts by binding the high affinity cell surface receptor, epidermal growth factor receptor. Defects in this gene are the cause of hypomagnesemia type 4. Dysregulation of this gene has been associated with the growth and progression of certain cancers. Alternate splicing results in multiple transcript variants.[provided by RefSeq, May 2010]
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Database

Research Area

SDS
PBS.pdf