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EGF antibody

Anti-EGF antibody used in ELISA (ELISA). GTX29695
Anti-EGF antibody used in IHC (Paraffin sections) (IHC-P). GTX29695
Anti-EGF antibody used in IHC (Paraffin sections) (IHC-P). GTX29695
Anti-EGF antibody used in IHC (Paraffin sections) (IHC-P). GTX29695
Anti-EGF antibody used in Western Blot (WB). GTX29695
Anti-EGF antibody used in Western Blot (WB). GTX29695

Cat. No. GTX29695

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA, Neutralizing/Inhibition, Sandwich ELISA

Reactivity

 Human
Package
50 μg ($399)
See all EGF products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.1 - 0.2 μg/mL
IHC-P Assay dependent
ELISA 0.1 - 0.2 μg/mL
Neutralizing/Inhibition 0.25 - 0.35 μg/mL
Sandwich ELISA 0.5 - 2.0 ug/ml

Note :

Neutralizing/Inhibition
To yield one-half maximal inhibition [ND₅₀] of the biological activity of hEGF (2.0 ng/mL)

Sandwich ELISA
Capture : GTX29695, Detection : Biotinylated Anti-Human EGF antibody
Not tested in other applications.

Calculated MW

134 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hEGF.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

epidermal growth factor , HOMG4 , URG

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

Epidermal Growth Factor (EGF) is a polypeptide growth factor, which stimulates the proliferation of a wide range of epidermal and epithelial cells.

Database

Research Area

DATA IMAGES

Anti-EGF antibody used in ELISA (ELISA). GTX29695

GTX29695 ELISA Image

ELISA analysis of human EGF recombinant protein(0.2 - 0.4 ng/well) using GTX29695 EGF antibody(capture antibody) at 0.5 - 2.0 ug/ml and Biotinylated Anti-Human EGF as a detection antibody.

Anti-EGF antibody used in IHC (Paraffin sections) (IHC-P). GTX29695

GTX29695 IHC-P Image

IHC-P analysis of human skin tissue using GTX29695 EGF antibody.
Working concentration : 1.0 ug/ml - 2.5 ug/ml
Antigen retrieval : proteinase K.

Anti-EGF antibody used in IHC (Paraffin sections) (IHC-P). GTX29695

GTX29695 IHC-P Image

IHC-P analysis of human skin tissue using GTX29695 EGF antibody.
Working concentration : 1.0 ug/ml - 2.5 ug/ml
Antigen retrieval : proteinase K.

Anti-EGF antibody used in IHC (Paraffin sections) (IHC-P). GTX29695

GTX29695 IHC-P Image

IHC-P analysis of human skin tissue using GTX29695 EGF antibody.
Working concentration : 1.0 ug/ml - 2.5 ug/ml
Antigen retrieval : proteinase K.

Anti-EGF antibody used in Western Blot (WB). GTX29695

GTX29695 WB Image

WB analysis of sequential diluted human EGF under either reducing or non-reducing conditions using GTX29695 EGF antibody.
Working concentration : 0.1 - 0.2 ug/ml

Anti-EGF antibody used in Western Blot (WB). GTX29695

GTX29695 WB Image

WB analysis of sequential diluted human EGF under either reducing or non-reducing conditions using GTX29695 EGF antibody.
Working concentration : 0.1 - 0.2 ug/ml

REFERENCE

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SDS
PBS.pdf
Package List Price ($)
$ 399