GeneTex
United States (US)

EGF antibody

GTX29695_2450_WB_20180221_18121410_822.jpg
GTX29695_855_IHC-P_20180221_18121410_327.jpg
GTX29695_2449_WB_20180221_18121410_147.jpg
GTX29695_854_IHC-P_20180221_18121410_494.jpg
GTX29695_9_ELISA_20180221_18121410_236.jpg
GTX29695_856_IHC-P_20180221_18121410_497.jpg

Cat No. GTX29695

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, ELISA, Neut, sELISA
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.1 - 0.2 μg/mL
IHC-P Assay dependent
ELISA 0.1 - 0.2 μg/mL
Neut 0.25 - 0.35 μg/mL
sELISA 0.5 - 2.0 ug/ml

Note :

Neut
To yield one-half maximal inhibition [ND50] of the biological activity of hEGF (2.0 ng/mL)

sELISA
Capture : GTX29695, Detection : Biotinylated Anti-Human EGF antibody

Not tested in other applications.

Calculated MW

134 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS pH 7.4

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hEGF.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

P01133, 1950, URG, 131530, EGF

Cellular Localization

Secreted

Background

Epidermal Growth Factor (EGF) is a polypeptide growth factor, which stimulates the proliferation of a wide range of epidermal and epithelial cells.

Database

Research Area

DATA IMAGES
GTX29695_2450_WB_20180221_18121410_822.jpg

GTX29695 WB Image

WB analysis of sequential diluted human EGF under either reducing or non-reducing conditions using GTX29695 EGF antibody.
Working concentration : 0.1 - 0.2 ug/ml

GTX29695_855_IHC-P_20180221_18121410_327.jpg

GTX29695 IHC-P Image

IHC-P analysis of human skin tissue using GTX29695 EGF antibody.
Working concentration : 1.0 ug/ml - 2.5 ug/ml
Antigen retrieval : proteinase K.

GTX29695_2449_WB_20180221_18121410_147.jpg

GTX29695 WB Image

WB analysis of sequential diluted human EGF under either reducing or non-reducing conditions using GTX29695 EGF antibody.
Working concentration : 0.1 - 0.2 ug/ml

GTX29695_854_IHC-P_20180221_18121410_494.jpg

GTX29695 IHC-P Image

IHC-P analysis of human skin tissue using GTX29695 EGF antibody.
Working concentration : 1.0 ug/ml - 2.5 ug/ml
Antigen retrieval : proteinase K.

GTX29695_9_ELISA_20180221_18121410_236.jpg

GTX29695 ELISA Image

ELISA analysis of human EGF recombinant protein(0.2 - 0.4 ng/well) using GTX29695 EGF antibody(capture antibody) at 0.5 - 2.0 ug/ml and Biotinylated Anti-Human EGF as a detection antibody.

GTX29695_856_IHC-P_20180221_18121410_497.jpg

GTX29695 IHC-P Image

IHC-P analysis of human skin tissue using GTX29695 EGF antibody.
Working concentration : 1.0 ug/ml - 2.5 ug/ml
Antigen retrieval : proteinase K.