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EGFR (E746-A750del) antibody [HL363]

Anti-EGFR (E746-A750del) antibody [HL363] used in Western Blot (WB). GTX635716

Cat No. GTX635716

Host

Rabbit

Clonality

Monoclonal

Clone Name

HL363

Isotype

IgG

Application

WB

Reactivity

Human
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

134 kDa. ( Note )

Product Note

This antibody is specific for EGFR E746-A750del mutant protein. It does not recognize EGFR wide type protein.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The immunogen used to generate this antibody corresponds to human EGFR (E746-A750del).

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2888572

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

epidermal growth factor receptor , ERBB , ERBB1 , HER1 , NISBD2 , PIG61 , mENA

Cellular Localization

Cell membrane,Endoplasmic reticulum membrane,Endosome,Endosome membrane,Nucleus

Background

The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq, Jun 2016]

Database

Research Area

DATA IMAGES

Anti-EGFR (E746-A750del) antibody [HL363] used in Western Blot (WB). GTX635716

GTX635716 WB Image

Various whole cell extracts were separated by 5% SDS-PAGE, and the membrane was blotted with EGFR (E746-A750del) antibody [HL363] (GTX635716) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
PC-9 cells express EGFR (Glu746-Ala750 deletion) protein and H1299 cells express wide type EGFR protein. (refer to PMID: 22157369)

REFERENCE

There are currently no references for EGFR (E746-A750del) antibody [HL363] (GTX635716). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for EGFR (E746-A750del) antibody [HL363] (GTX635716). Be the first to share your experience with this product.
Package List Price ($)
$ 379
$ 169
Custom Conjugations