EGFR VIII antibody [HL2541]

Cat. No. GTX638910

Host	Rabbit
Clonality	Monoclonal
Clone Name	HL2541
Isotype	IgG
Application	WB, ICC/IF, IHC-P (cell pellet)
Reactivity	Human
Package	100 μl ($399), 25 μl ($169)

See all EGFR VIII products

Datasheet

View product citations for antibody GTX638910 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	Assay dependent
IHC-P (cell pellet)	Assay dependent

Not tested in other applications.

Calculated MW

134 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide of human EGFR VIII.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

EGFVviii , EGFR mutant , EGFR variant , EGFRv3

Cellular Localization

Cell membrane,Endoplasmic reticulum membrane,Endosome,Endosome membrane,Nucleus,Secreted

Background

The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq, Jun 2016]

Database

Gene ID: 1956 EGFR
UniProt: P00533 EGFR

Research Area

DATA IMAGES

GTX638910 ICC/IF Image

EGFR VIII antibody [HL2541] detects EGFR VIII protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: EGFR VIII stained by EGFR VIII antibody [HL2541] (GTX638910) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX638910 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EGFR VIII antibody [HL2541] (GTX638910) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX638910 IHC-P (cell pellet) Image

EGFR VIII antibody [HL2541] detects EGFR VIII protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mock and transfected 293T cell pellet.
Green: EGFR VIII stained by EGFR VIII antibody [HL2541] (GTX638910) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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Package

List Price ($)

100 μl

$ 399

25 μl

$ 169

Qty Custom Conjugations