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EGFR antibody [C2C3], C-term

KO/KD IP/MS
GTX100448_39645_20160630_WB_shRNA_watermark_19011506_142.jpg
GTX100448_39645_IHC_2_19011506_831.jpg
GTX100448_39645_IHC_19011506_752.jpg
GTX100448_39645_IP_19011506_428.jpg
GTX100448_39645_20180601_WB_19011506_188.jpg
GTX100448_39645_IFA_19011506_623.jpg

Cat No. GTX100448

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, FACS, IP
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
FACS Assay dependent
IP 1:100-1:500
Not tested in other applications.

Calculated MW

134 kDa. ( Note )

Observed MW

180 kDa.

Positive Control

A431 , A549 , H1299 , HCT116 , *Beas-2 , *SiHa , *Hela , *Huh7 , *MSP

Specificity/Sensitivity

IP/MS validation was supported by references (PMID:30377372)

Predict Reactivity

Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 40% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human EGFR. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

ERBB antibody, ERBB1 antibody, HER1 antibody, PIG61 antibody, mENA antibody, EGFR antibody, receptor tyrosine-protein kinase erbB-1 antibody, cell growth inhibiting protein 40 antibody, epidermal growth factor receptor antibody, proto-oncogene c-ErbB-1 antibody, avian erythroblastic leukemia viral (v-erb-b) oncogene homolog antibody, cell proliferation-inducing protein 61 antibody

Cellular Localization

Cell membrane; Single-pass type I membrane protein , Isoform 2: Secreted

Background

The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX100448_39645_20160630_WB_shRNA_watermark_19011506_142.jpg

GTX100448 WB Image

KO/KD

Non-transfected (–) and transfected (+) A431 whole cell extracts (15 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with EGFR antibody [C2C3], C-term (GTX100448) diluted at 1:6000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100448_39645_IHC_2_19011506_831.jpg

GTX100448 IHC-P Image

EGFR antibody [C2C3], C-term detects EGFR protein at membrane on human gastric cancer by immunohistochemical analysis.
Sample: Paraffin-embedded gastric cancer.
EGFR antibody [C2C3], C-term (GTX100448) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100448_39645_IHC_19011506_752.jpg

GTX100448 IHC-P Image

EGFR antibody [C2C3], C-term detects EGFR protein at membrane on human gastric cancer by immunohistochemical analysis.
Sample: Paraffin-embedded gastric cancer .
EGFR antibody [C2C3], C-term (GTX100448) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100448_39645_IP_19011506_428.jpg

GTX100448 IP Image

EGFR antibody [C2C3], C-term immunoprecipitates EGFR protein in IP experiments.
IP samples: A431 whole cell extract
A. 40 μg A431 whole cell extract
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of EGFR protein by 4 μg EGFR antibody [C2C3], C-term (GTX100448)
5 % SDS-PAGE
The immunoprecipitated EGFR protein was detected by EGFR antibody [C2C3], C-term (GTX100448) diluted at 1:1000.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

GTX100448_39645_20180601_WB_19011506_188.jpg

GTX100448 WB Image

Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with EGFR antibody [C2C3], C-term (GTX100448) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100448_39645_IFA_19011506_623.jpg

GTX100448 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed A431, using EGFR(GTX100448) antibody (Green) at 1:1000 dilution.

REFERENCE
REVIEW

EGFR antibody [C2C3], C-term Cat No. GTX100448

Rating ( Average 4 based on 7 users reviews)
Application
Western Blot(WB) ( Average 4.3 based on 4 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 4 based on 1 users reviews)
Flow cytometry(FACS) ( Average 3.5 based on 2 users reviews)
Date : Anonymous submitted on 21-Sep-2016
Score :
Application Tested : WB
Sample Species : Hu
Sample : Huh-7 cells
Amount used : 15μg
Blocking : 5% milk in PBST, 25°C, 2Hr
Primary Antibody : 1:3000, 4°C, overnightHr
Date : Anonymous submitted on 18-Jul-2013
Score :
Application Tested : FACS
Sample Species : Hu
Sample : MSP cells
Blocking : 02.% FBS in PBS, 4°C, 0.17Hr
Primary Antibody : 1:250, 4°C, 1Hr
Date : Anonymous submitted on 25-Jun-2013
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : A549
Blocking : 3% BSA, 25°C, 0.5Hr
Fixation : 2.5% paraformaldehyde, 0.5Hr
Primary Antibody : 1:100, 25°C, 1Hr
Date : Anonymous submitted on 27-Mar-2013
Score :
Application Tested : FACS
Sample Species : Hu
Sample : Huh7 cells
Permeabilization /Antigen retrieval : 1%BSA/PBS
Primary Antibody : 1:100, 25°C, 0.5Hr
Date : Anonymous submitted on 30-Jan-2012
Score :
Application Tested : WB
Sample Species : Hu
Sample : A549 cells,SiHa cells,HeLa cells
Amount used : 10μg
Blocking : 5% milk, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, overnightHr
Date : Anonymous submitted on 11-Jan-2012
Score :
Application Tested : WB
Sample Species : Hu
Sample : A431 cells,A549 cells
Amount used : 50μg
Blocking : 5% non-fat milk in TBST, 25°C, 16Hr
Primary Antibody : 1:8000, 25°C, 3Hr
Date : Anonymous submitted on 21-Oct-2011
Score :
Application Tested : WB
Sample Species : Hu
Sample : Beas-2b cells
Amount used : 40μg
Blocking : 3% BSA in PBST, 25°C, 1Hr
Primary Antibody : 1:2000, 25°C, 2Hr
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169