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EGFR antibody [C2C3], C-term

KO/KD Orthogonal IP/MS
Anti-EGFR antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100448
Anti-EGFR antibody [C2C3], C-term used in Western Blot (WB). GTX100448
Anti-EGFR antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100448
Anti-EGFR antibody [C2C3], C-term used in Immunoprecipitation (IP). GTX100448
Anti-EGFR antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100448
Anti-EGFR antibody [C2C3], C-term used in Western Blot (WB). GTX100448
Anti-EGFR antibody [C2C3], C-term used in Western Blot (WB). GTX100448

Cat. No. GTX100448

Reference 5
7

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, FACS, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all EGFR products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
FACS Assay dependent
IP 1:100-1:500
Not tested in other applications.

Calculated MW

134 kDa. ( Note )

Observed MW

180 kDa.

Positive Control

A431

Product Note

IP/MS validation was supported by references (PMID:30377372)

Predict Reactivity

Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.28 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Intracellular domain of human EGFR. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950176

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

epidermal growth factor receptor , ERBB , ERBB1 , HER1 , NISBD2 , PIG61 , mENA

Cellular Localization

Cell membrane; Single-pass type I membrane protein , Isoform 2: Secreted

Background

The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-EGFR antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100448

GTX100448 IHC-P Image

EGFR antibody [C2C3], C-term detects EGFR protein at membrane on human gastric cancer by immunohistochemical analysis.
Sample: Paraffin-embedded gastric cancer.
EGFR antibody [C2C3], C-term (GTX100448) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-EGFR antibody [C2C3], C-term used in Western Blot (WB). GTX100448

GTX100448 WB Image

KO/KD

Non-transfected (–) and transfected (+) A431 whole cell extracts (15 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with EGFR antibody [C2C3], C-term (GTX100448) diluted at 1:6000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-EGFR antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100448

GTX100448 IHC-P Image

EGFR antibody [C2C3], C-term detects EGFR protein at membrane on human gastric cancer by immunohistochemical analysis.
Sample: Paraffin-embedded gastric cancer .
EGFR antibody [C2C3], C-term (GTX100448) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-EGFR antibody [C2C3], C-term used in Immunoprecipitation (IP). GTX100448

GTX100448 IP Image

EGFR antibody [C2C3], C-term immunoprecipitates EGFR protein in IP experiments.
IP samples: A431 whole cell extract
A. 40 μg A431 whole cell extract
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of EGFR protein by 4 μg EGFR antibody [C2C3], C-term (GTX100448)
5 % SDS-PAGE
The immunoprecipitated EGFR protein was detected by EGFR antibody [C2C3], C-term (GTX100448) diluted at 1:1000.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
Anti-EGFR antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100448

GTX100448 ICC/IF Image

EGFR antibody [C2C3], C-term detects EGFR protein at cell membrane by immunofluorescent analysis.
Sample: A431 cells were fixed in ice-cold MeOH for 5 min.
Green: EGFR stained by EGFR antibody [C2C3], C-term (GTX100448) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-EGFR antibody [C2C3], C-term used in Western Blot (WB). GTX100448

GTX100448 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with EGFR antibody [C2C3], C-term (GTX100448) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-EGFR antibody [C2C3], C-term used in Western Blot (WB). GTX100448

GTX100448 WB Image

Orthogonal

The data was published in the 2017 in Oxid Med Cell Longev. PMID: 28740570

REFERENCE

Application Reference

Abdi K et al. Cell Rep 2019; 28 (8) : 2012-2022.e4 EGFR Signaling Termination via Numb Trafficking in Ependymal Progenitors Controls Postnatal Neurogenic Niche Differentiation.
Application : WB, IP
Reactivity : Mouse
Chen CF et al. Oncol Lett 2018; 16 (5) : 6323-6330
Sikorski K et al. Nat Methods 2018; 15 (11) : 909-912 A high-throughput pipeline for validation of antibodies.
Application : IP
Reactivity : Human
Carrasco-Torres G et al. Oxid Med Cell Longev 2017; 2017 : 4674918 Quercetin reverses rat liver preneoplastic lesions induced by chemical carcinogenesis.
Application : WB
Reactivity : Rat
Liu CH et al. Cell Transplant 2014; 23 (43195) : 657-68 Salvianolic Acid B Maintained Stem Cell Pluripotency and Increased Proliferation Rate by Activating Jak2-Stat3 Combined With EGFR-Erk1/2 Pathways.
Application : WB
Reactivity : Mouse
Submit a Reference

REVIEW

EGFR antibody [C2C3], C-term Cat. No. GTX100448

Rating
( Average 4 based on 7 users reviews)
Application
Western Blot(WB)
( Average 4.3 based on 4 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF)
( Average 4 based on 1 users reviews)
Flow cytometry(FACS)
( Average 3.5 based on 2 users reviews)
Anti-EGFR antibody [C2C3], C-term used in Western Blot (WB). GTX100448
Date : Anonymous submitted on 21-Sep-2016
Score :
Application Tested : WB
Sample Species : Hu
Sample : Huh-7 cells
Amount used : 15μg
Blocking : 5% milk in PBST, 25°C, 2Hr
Primary Antibody : 1:3000, 4°C, 16Hr
Anti-EGFR antibody [C2C3], C-term used in Flow cytometry (FACS). GTX100448
Date : Anonymous submitted on 18-Jul-2013
Score :
Application Tested : FACS
Sample Species : Hu
Sample : MSP cells
Blocking : 02.% FBS in PBS, 4°C, 0.17Hr
Primary Antibody : 1:250, 4°C, 1Hr
Anti-EGFR antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100448
Date : Anonymous submitted on 25-Jun-2013
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : A549
Blocking : 3% BSA, 25°C, 0.5Hr
Fixation : 2.5% paraformaldehyde, 0.5Hr
Primary Antibody : 1:100, 25°C, 1Hr
Anti-EGFR antibody [C2C3], C-term used in Flow cytometry (FACS). GTX100448
Date : Anonymous submitted on 27-Mar-2013
Score :
Application Tested : FACS
Sample Species : Hu
Sample : Huh7 cells
Permeabilization /Antigen retrieval : 1%BSA/PBS
Primary Antibody : 1:100, 25°C, 0.5Hr
Anti-EGFR antibody [C2C3], C-term used in Western Blot (WB). GTX100448
Date : Anonymous submitted on 30-Jan-2012
Score :
Application Tested : WB
Sample Species : Hu
Sample : A549 cells,SiHa cells,HeLa cells
Amount used : 10μg
Blocking : 5% milk, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Anti-EGFR antibody [C2C3], C-term used in Western Blot (WB). GTX100448
Date : Anonymous submitted on 11-Jan-2012
Score :
Application Tested : WB
Sample Species : Hu
Sample : A431 cells,A549 cells
Amount used : 50μg
Blocking : 5% non-fat milk in TBST, 25°C, 16Hr
Primary Antibody : 1:8000, 25°C, 3Hr
Anti-EGFR antibody [C2C3], C-term used in Western Blot (WB). GTX100448
Date : Anonymous submitted on 21-Oct-2011
Score :
Application Tested : WB
Sample Species : Hu
Sample : Beas-2b cells
Amount used : 40μg
Blocking : 3% BSA in PBST, 25°C, 1Hr
Primary Antibody : 1:2000, 25°C, 2Hr
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169