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EGFR antibody [GT133]

KO/KD Comparison Orthogonal Overexpression IP/MS
Anti-EGFR antibody [GT133] used in Western Blot (WB). GTX628887
Anti-EGFR antibody [GT133] used in Western Blot (WB). GTX628887
Anti-EGFR antibody [GT133] used in Western Blot (WB). GTX628887
Anti-EGFR antibody [GT133] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628887
Anti-EGFR antibody [GT133] used in Western Blot (WB). GTX628887
Anti-EGFR antibody [GT133] used in IHC (Paraffin sections) (IHC-P). GTX628887
Anti-EGFR antibody [GT133] used in IHC (Paraffin sections) (IHC-P). GTX628887
Anti-EGFR antibody [GT133] used in Western Blot (WB). GTX628887
Anti-EGFR antibody [GT133] used in Immunoprecipitation (IP). GTX628887
Anti-EGFR antibody [GT133] used in Western Blot (WB). GTX628887

Cat. No. GTX628887

Reference 4

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT133

Isotype

 IgG2b

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all EGFR products
PRODUCT

Summary

EGFR antibody recognizes epidermal growth factor receptor (EGFR, also known as HER1 in humans), a receptor tyrosine kinase with a predicted molecular weight of 134 kDa. EGFR is involved in epithelial tissue development and homeostasis. It is also a driver of tumorigenesis. Upregulation or abnormal activation of EGFR is frequently found in glioblastomas, lung cancers, and breast cancers, among others.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

134 kDa. ( Note )

Observed MW

180 kDa.

Positive Control

A431 , A549 , H1299 , HCT116

Product Note

This antibody was raised against human EGFR Intracellular domain.This antibody does not cross-react with Her2/Erbb2 protein.
IP/MS validation was supported by references (PMID:30377423)

Predict Reactivity

Mouse, Rat, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Intracellular domain of human EGFR. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888064

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

epidermal growth factor receptor , ERBB , ERBB1 , HER1 , NISBD2 , PIG61 , mENA

Cellular Localization

Cell membrane; Single-pass type I membrane protein , Isoform 2: Secreted

Background

The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-EGFR antibody [GT133] used in Western Blot (WB). GTX628887

GTX628887 WB Image

KO/KD

Non-transfected (–) and transfected (+) A431 whole cell extracts (15 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with EGFR antibody [GT133] (GTX628887) diluted at 1:8000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-EGFR antibody [GT133] used in Western Blot (WB). GTX628887

GTX628887 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts were separated by 5% SDS-PAGE, and the membrane was blotted with EGFR antibody [GT133] (GTX628887) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-EGFR antibody [GT133] used in Western Blot (WB). GTX628887

GTX628887 WB Image

Comparison

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membranes were blotted with EGFR antibody [GT133] (GTX628887) diluted at 1:5000 and competitor's antibody diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-EGFR antibody [GT133] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628887

GTX628887 ICC/IF Image

EGFR antibody [GT133] detects EGFR protein at cell membrane by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: EGFR stained by EGFR antibody [GT133] (GTX628887) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-EGFR antibody [GT133] used in Western Blot (WB). GTX628887

GTX628887 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with EGFR antibody [GT133] (GTX628887) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-EGFR antibody [GT133] used in IHC (Paraffin sections) (IHC-P). GTX628887

GTX628887 IHC-P Image

EGFR antibody [GT133] detects EGFR protein at cell membrane on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human colon carcinoma.
EGFR antibody [GT133] (GTX628887) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-EGFR antibody [GT133] used in IHC (Paraffin sections) (IHC-P). GTX628887

GTX628887 IHC-P Image

EGFR antibody [GT133] detects EGFR protein at cell membrane on human ovarian carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian carcinoma.
EGFR antibody [GT133] (GTX628887) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-EGFR antibody [GT133] used in Western Blot (WB). GTX628887

GTX628887 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with EGFR antibody [GT133] (GTX628887) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-EGFR antibody [GT133] used in Immunoprecipitation (IP). GTX628887

GTX628887 IP Image

EGFR antibody [GT133] immunoprecipitates EGFR protein in IP experiments.
IP samples: A431 whole cell extract
A. 40 μg A431 whole cell extract
B. Control with 4 μg of preimmune Mouse IgG
C. Immunoprecipitation of EGFR protein by 4 μg EGFR antibody [GT133] (GTX628887)
5 % SDS-PAGE
The immunoprecipitated EGFR protein was detected by EGFR antibody [GT133] (GTX628887) diluted at 1:5000.
[EasyBlot anti-mouse IgG (GTX221667-01) was used as a secondary reagent]
Anti-EGFR antibody [GT133] used in Western Blot (WB). GTX628887

GTX628887 WB Image

Orthogonal

The data was published in the 2022 in J Oncol. PMID: 35646111

REFERENCE

Application Reference

Pang HH et al. ACS Nano 2023; 17 (11) : 10407-10422 Bioengineered Bacteriophage-Like Nanoparticles as RNAi Therapeutics to Enhance Radiotherapy against Glioblastomas.
Ching-Ann Liu et al. J Oncol 2022; 2022 : 3236058 Targeting the Axl and mTOR Pathway Synergizes Immunotherapy and Chemotherapy to Butylidenephthalide in a Recurrent GBM.
Sikorski K et al. Nat Methods 2018; 15 (11) : 909-912 A high-throughput pipeline for validation of antibodies.
Application : IP
Reactivity : Human
Baykul T et al. J Res Med Den Sci 2016; 4 (1) : 25-29 EGFR Expression and Incidence of Cystic Changes in Asymptomatic Dental Follicles in Smoking Patients.
Application : IHC-P
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations