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EGFR antibody [GT133]

KO/KD Comparison Orthogonal Overexpression IP/MS
Anti-EGFR antibody [GT133] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628887
Anti-EGFR antibody [GT133] used in Western Blot (WB). GTX628887
Anti-EGFR antibody [GT133] used in Western Blot (WB). GTX628887
Anti-EGFR antibody [GT133] used in Western Blot (WB). GTX628887
Anti-EGFR antibody [GT133] used in IHC (Paraffin sections) (IHC-P). GTX628887
Anti-EGFR antibody [GT133] used in Western Blot (WB). GTX628887
Anti-EGFR antibody [GT133] used in Immunoprecipitation (IP). GTX628887
Anti-EGFR antibody [GT133] used in IHC (Paraffin sections) (IHC-P). GTX628887
Anti-EGFR antibody [GT133] used in Western Blot (WB). GTX628887

Cat No. GTX628887

Reference 2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT133

Isotype

 IgG2b

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human
Package
100 μl ($329),
25 μl ($169)
See all EGFR products
PRODUCT

Summary

EGFR antibody recognizes epidermal growth factor receptor (EGFR, also known as HER1 in humans), a receptor tyrosine kinase with a predicted molecular weight of 134 kDa. EGFR is involved in epithelial tissue development and homeostasis. It is also a driver of tumorigenesis. Upregulation or abnormal activation of EGFR is frequently found in glioblastomas, lung cancers, and breast cancers, among others.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

134 kDa. ( Note )

Observed MW

180 kDa.

Positive Control

A431 , A549 , H1299 , HCT116

Product Note

This antibody does not cross-react with Her2/Erbb2 protein.
IP/MS validation was supported by references (PMID:30377423)

Predict Reactivity

Mouse, Rat, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human EGFR. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888064

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

epidermal growth factor receptor , ERBB , ERBB1 , HER1 , NISBD2 , PIG61 , mENA

Cellular Localization

Cell membrane; Single-pass type I membrane protein , Isoform 2: Secreted

Background

The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-EGFR antibody [GT133] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628887

GTX628887 ICC/IF Image

EGFR antibody [GT133] detects EGFR protein at cell membrane by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde for 10 min.
Green: EGFR protein stained by EGFR antibody [GT133] (GTX628887) diluted at 1:300.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-EGFR antibody [GT133] used in Western Blot (WB). GTX628887

GTX628887 WB Image

KO/KD

Non-transfected (–) and transfected (+) A431 whole cell extracts (15 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with EGFR antibody [GT133] (GTX628887) diluted at 1:8000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-EGFR antibody [GT133] used in Western Blot (WB). GTX628887

GTX628887 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts were separated by 5% SDS-PAGE, and the membrane was blotted with EGFR antibody [GT133] (GTX628887) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-EGFR antibody [GT133] used in Western Blot (WB). GTX628887

GTX628887 WB Image

Comparison

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membranes were blotted with EGFR antibody [GT133] (GTX628887) diluted at 1:5000 and competitor's antibody diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-EGFR antibody [GT133] used in IHC (Paraffin sections) (IHC-P). GTX628887

GTX628887 IHC-P Image

EGFR antibody [GT133] detects EGFR protein at cell membrane on human ovarian carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian carcinoma.
EGFR antibody [GT133] (GTX628887) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-EGFR antibody [GT133] used in Western Blot (WB). GTX628887

GTX628887 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with EGFR antibody [GT133] (GTX628887) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-EGFR antibody [GT133] used in Immunoprecipitation (IP). GTX628887

GTX628887 IP Image

EGFR antibody [GT133] immunoprecipitates EGFR protein in IP experiments.
IP samples: A431 whole cell extract
A. 40 μg A431 whole cell extract
B. Control with 4 μg of preimmune Mouse IgG
C. Immunoprecipitation of EGFR protein by 4 μg EGFR antibody [GT133] (GTX628887)
5 % SDS-PAGE
The immunoprecipitated EGFR protein was detected by EGFR antibody [GT133] (GTX628887) diluted at 1:5000.
[EasyBlot anti-mouse IgG (GTX221667-01) was used as a secondary reagent]
Anti-EGFR antibody [GT133] used in IHC (Paraffin sections) (IHC-P). GTX628887

GTX628887 IHC-P Image

EGFR antibody [GT133] detects EGFR protein at cell membrane on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human colon carcinoma.
EGFR antibody [GT133] (GTX628887) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-EGFR antibody [GT133] used in Western Blot (WB). GTX628887

GTX628887 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with EGFR antibody [GT133] (GTX628887) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

REFERENCE

Application Reference

Sikorski K et al. Nat Methods 2018; 15 (11) : 909-912 A high-throughput pipeline for validation of antibodies.
Application : IP
Reactivity : Human
Baykul T et al. J Res Med Den Sci 2016; 4 (1) : 25-29 EGFR Expression and Incidence of Cystic Changes in Asymptomatic Dental Follicles in Smoking Patients.
Application : IHC-P
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Package List Price ($)
$ 329
$ 169