PRODUCT
Summary
EGFR antibody recognizes epidermal growth factor receptor (EGFR, also known as HER1 in humans), a receptor tyrosine kinase with a predicted molecular weight of 134 kDa. EGFR is involved in epithelial tissue development and homeostasis. It is also a driver of tumorigenesis. Upregulation or abnormal activation of EGFR is frequently found in glioblastomas, lung cancers, and breast cancers, among others.
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:5000-1:20000 |
1:100-1:1000 |
1:100-1:1000 |
1:100-1:500 |
Not tested in other applications.
Calculated MW
Observed MW
180 kDa.
Positive Control
A431 , A549 , H1299 , HCT116
Product Note
This antibody does not cross-react with Her2/Erbb2 protein.
IP/MS validation was supported by references (PMID:30377423)
Predict Reactivity
Mouse, Rat, Pig(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No Preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human EGFR. The exact sequence is proprietary.
Purification
Affinity purified by Protein G.
Conjugation
Unconjugated
RRID
AB_2888064
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
epidermal growth factor receptor , ERBB , ERBB1 , HER1 , NISBD2 , PIG61 , mENA
Cellular Localization
Cell membrane; Single-pass type I membrane protein , Isoform 2: Secreted
Background
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX628887 WB Image
Non-transfected (–) and transfected (+) A431 whole cell extracts (15 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with EGFR antibody [GT133] (GTX628887) diluted at 1:8000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX628887 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts were separated by 5% SDS-PAGE, and the membrane was blotted with EGFR antibody [GT133] (GTX628887) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX628887 WB Image
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membranes were blotted with EGFR antibody [GT133] (GTX628887) diluted at 1:5000 and competitor's antibody diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
*The competitor is not affiliated with GeneTex and does not endorse this product.
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GTX628887 WB Image
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with EGFR antibody [GT133] (GTX628887) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX628887 IHC-P Image
EGFR antibody [GT133] detects EGFR protein at cell membrane on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human colon carcinoma.
EGFR antibody [GT133] (GTX628887) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX628887 IHC-P Image
EGFR antibody [GT133] detects EGFR protein at cell membrane on human ovarian carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian carcinoma.
EGFR antibody [GT133] (GTX628887) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX628887 WB Image
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with EGFR antibody [GT133] (GTX628887) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
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GTX628887 ICC/IF Image
EGFR antibody [GT133] detects EGFR protein at cell membrane by immunofluorescent analysis.Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: EGFR stained by EGFR antibody [GT133] (GTX628887) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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GTX628887 IP Image
EGFR antibody [GT133] immunoprecipitates EGFR protein in IP experiments. IP samples: A431 whole cell extract A. 40 μg A431 whole cell extract B. Control with 4 μg of preimmune Mouse IgG C. Immunoprecipitation of EGFR protein by 4 μg EGFR antibody [GT133] (GTX628887) 5 % SDS-PAGE The immunoprecipitated EGFR protein was detected by EGFR antibody [GT133] (GTX628887) diluted at 1:5000. [EasyBlot anti-mouse IgG (GTX221667-01) was used as a secondary reagent]
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REFERENCE
REVIEW
EGFR antibody [GT133]
Cat. No. GTX628887
Rating
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( Average 5 based on 1 users reviews)
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Application
Western Blot(WB)
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( Average 5 based on 1 users reviews)
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Date :
Anonymous submitted on 24-Jul-2023
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the 2022 in J Oncol.
PMID: 35646111