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EGFR antibody [GT461]

Anti-EGFR antibody [GT461] used in Western Blot (WB). GTX628888
Anti-EGFR antibody [GT461] used in Western Blot (WB). GTX628888
Anti-EGFR antibody [GT461] used in Western Blot (WB). GTX628888
Anti-EGFR antibody [GT461] used in Western Blot (WB). GTX628888
Anti-EGFR antibody [GT461] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628888
Anti-EGFR antibody [GT461] used in Immunoprecipitation (IP). GTX628888

Cat No. GTX628888

Host

Mouse

Clonality

Monoclonal

Clone Name

GT461

Isotype

IgG2a

Application

WB, ICC/IF, IHC-P, IP

Reactivity

Human
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P Assay dependent
IP 1:100-1:500
Not tested in other applications.

Calculated MW

134 kDa. ( Note )

Observed MW

180 kDa.

Positive Control

A431 , A549

Product Note

IP/MS validation was supported by references (PMID:30377424)

Predict Reactivity

Mouse, Rat, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.01 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human EGFR. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888065

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

epidermal growth factor receptor , ERBB , ERBB1 , HER1 , NISBD2 , PIG61 , mENA

Cellular Localization

Cell membrane; Single-pass type I membrane protein , Isoform 2: Secreted

Background

The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-EGFR antibody [GT461] used in Western Blot (WB). GTX628888

GTX628888 WB Image

Non-transfected (–) and transfected (+) A431 whole cell extracts (15 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with EGFR antibody [GT461] (GTX628888) diluted at 1:8000.

Anti-EGFR antibody [GT461] used in Western Blot (WB). GTX628888

GTX628888 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with EGFR antibody [GT461] (GTX628888) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-EGFR antibody [GT461] used in Western Blot (WB). GTX628888

GTX628888 WB Image

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with EGFR antibody [GT461] (GTX628888) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-EGFR antibody [GT461] used in Western Blot (WB). GTX628888

GTX628888 WB Image

EGFR antibody [GT461] detects EGFR protein by Western blot analysis.
A. 30 μg A431 whole cell lysate/extract
5 % SDS-PAGE
EGFR antibody [GT461] (GTX628888) dilution: 1:10000

Anti-EGFR antibody [GT461] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628888

GTX628888 ICC/IF Image

EGFR antibody [GT461] detects EGFR protein at cell membrane by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde for 10 min.
Green: EGFR protein stained by EGFR antibody [GT461] (GTX628888) diluted at 1:300.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-EGFR antibody [GT461] used in Immunoprecipitation (IP). GTX628888

GTX628888 IP Image

Immunoprecipitation of EGFR protein from A431 whole cell extracts using 5 μg of EGFR antibody [GT461] (GTX628888).
Western blot analysis was performed using EGFR antibody [GT461] (GTX628888).
EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent.

REFERENCE

REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 379
$ 169
Custom Conjugations