EGFR antibody [HL1097]

EGFR antibody [HL1097] detects EGFR protein by immunohistochemical analysis.
Sample: Paraffin-embedded human tissues.
EGFR stained by EGFR antibody [HL1097] (GTX642543) diluted at 1:600.
Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min

EGFR antibody [HL1097] detects EGFR protein by immunofluorescent analysis.
Sample: A431 cells were fixed in ice-cold MeOH for 5 min.
Green: EGFR stained by EGFR antibody [HL1097] (GTX642543) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with EGFR antibody [HL1097] (GTX642543) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data are based on NCBI database.

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with EGFR antibody [C2C3], C-term (GTX642543) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data are based on Human Protein Atlas program.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 | 36 | 50 μg) were separated by 5% SDS-PAGE, and the membranes were blotted with EGFR antibody [HL1097] (GTX642543) diluted at 1:5000 and Her2 / ErbB2 antibody(GTX100509) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.