EGFR antibody [HL3325] detects EGFR protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
EGFR stained by EGFR antibody [HL3325] (GTX641090) diluted at 1:250.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Corresponding RNA levels (RPKM) in the tissues are based on NCBI database.

EGFR antibody [HL3325] detects EGFR protein by immunohistochemical analysis.
Sample: Paraffin-embedded human tissues.
EGFR stained by EGFR antibody [HL3325] (GTX641090) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Corresponding RNA levels (nTPM) in the tissues are based on Human Protein Atlas program.

Mouse tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with EGFR antibody [HL3325] (GTX641090) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Non-transfected (–) and transfected (+) 293T whole cell extracts were separated by 5% SDS-PAGE, and the membrane was blotted with EGFR antibody [HL3325] (GTX641090) and Her2 / ErbB2 antibody (GTX100509) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with EGFR antibody [HL3325] (GTX641090) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.