EGFR antibody [ICR10] (FITC)

Cat. No. GTX11400

Host	Rat
Clonality	Monoclonal
Clone Name	ICR10
Isotype	IgG2a
Application	ICC/IF, FACS
Reactivity	Human
Package	50 μg ($399)

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View product citations for antibody GTX11400 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
ICC/IF	Assay dependent
FACS	1/10
Note : FACS Use 10μl of the suggested working dilution to label 10⁶ cells in 100μl.

Not tested in other applications.

Calculated MW

134 kDa. ( Note )

Product Note

This antibody, clone ICR10 binds to epitope B from EGFR

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. Protect from light.

Concentration

0.1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Extracellular domain of human EGF-receptor from head and neck carcinoma.

Purification

Purified by ion exchange chromatography

Conjugation

Fluorescein isothiocyanate (FITC)

RRID

AB_368217

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

epidermal growth factor receptor , ERBB , ERBB1 , HER1 , NISBD2 , PIG61 , mENA

Cellular Localization

Cell membrane , Endoplasmic reticulum membrane , Endosome , Endosome membrane , Nucleus

Background

The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq, Jun 2016]