GeneTex

  • Country / Location Selection

United States (US)

EGFR antibody [N1-2], N-term

KO/KD Comparison Orthogonal
Anti-EGFR antibody [N1-2], N-term used in Western Blot (WB). GTX121919
Anti-EGFR antibody [N1-2], N-term used in IHC (Paraffin sections) (IHC-P). GTX121919
Anti-EGFR antibody [N1-2], N-term used in IHC (Paraffin sections) (IHC-P). GTX121919
Anti-EGFR antibody [N1-2], N-term used in Western Blot (WB). GTX121919
Anti-EGFR antibody [N1-2], N-term used in Western Blot (WB). GTX121919
Anti-EGFR antibody [N1-2], N-term used in Immunoprecipitation (IP). GTX121919
Anti-EGFR antibody [N1-2], N-term used in Western Blot (WB). GTX121919
Anti-EGFR antibody [N1-2], N-term used in Western Blot (WB). GTX121919
Anti-EGFR antibody [N1-2], N-term used in Western Blot and Immunoprecipitation (WB IP). GTX121919
Anti-EGFR antibody [N1-2], N-term used in Western Blot and Immunoprecipitation (WB IP). GTX121919
Anti-EGFR antibody [N1-2], N-term used in Western Blot (WB). GTX121919
Anti-EGFR antibody [N1-2], N-term used in Western Blot (WB). GTX121919
Anti-EGFR antibody [N1-2], N-term used in Western Blot and Immunoprecipitation (WB IP). GTX121919
Anti-EGFR antibody [N1-2], N-term used in Western Blot (WB). GTX121919

Cat. No. GTX121919

Reference 23
2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, FACS, IP, PLA

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all EGFR products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
FACS Assay dependent
IP 1:100-1:500
PLA Assay dependent
Not tested in other applications.

Calculated MW

134 kDa. ( Note )

Observed MW

180 kDa.

Positive Control

Mouse brain , A431 , HCT116

Predict Reactivity

Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.04 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Extracellular domain of human EGFR. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10729577

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

epidermal growth factor receptor , ERBB , ERBB1 , HER1 , NISBD2 , PIG61 , mENA

Cellular Localization

Cell membrane; Single-pass type I membrane protein , Isoform 2: Secreted

Background

The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-EGFR antibody [N1-2], N-term used in Western Blot (WB). GTX121919

GTX121919 WB Image

KO/KD

Non-transfected (–) and transfected (+) A431 whole cell extracts (15 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with EGFR antibody [N1-2], N-term (GTX121919) diluted at 1:12000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-EGFR antibody [N1-2], N-term used in IHC (Paraffin sections) (IHC-P). GTX121919

GTX121919 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using EGFR(GTX121919) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-EGFR antibody [N1-2], N-term used in IHC (Paraffin sections) (IHC-P). GTX121919

GTX121919 IHC-P Image

EGFR antibody [N1-2], N-term detects EGFR protein at cell membrane in human non-small cell lung cancer by immunohistochemical analysis.
Sample: Two independent paraffin-embedded human non-small cell lung cancer.
EGFR antibody [N1-2], N-term (GTX121919) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-EGFR antibody [N1-2], N-term used in Western Blot (WB). GTX121919

GTX121919 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with EGFR antibody [N1-2], N-term (GTX121919) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-EGFR antibody [N1-2], N-term used in Western Blot (WB). GTX121919

GTX121919 WB Image

Comparison

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membranes were blotted with EGFR antibody [N1-2], N-term (GTX121919) diluted at 1:500 and competitor's antibody (UM500070) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-EGFR antibody [N1-2], N-term used in Immunoprecipitation (IP). GTX121919

GTX121919 IP Image

EGFR protein was immunoprecipitated from A549 cell lysate using 5 ug of anti-EGFR antibody (GTX121919). The precipitated EGFR protein was detected by GTX121919 diluted at 1:500, and used EasyBlot anti-rabbit IgG (GTX221666-01) as secondary reagent.
A: A549 cell lysate.
B: IP by EGFR antibody.
7.5% SDS PAGE

Anti-EGFR antibody [N1-2], N-term used in Western Blot (WB). GTX121919

GTX121919 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with EGFR antibody [N1-2], N-term (GTX121919) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-EGFR antibody [N1-2], N-term used in Western Blot (WB). GTX121919

GTX121919 WB Image

Sample (50 μg of whole cell lysate)
A: Mouse brain
5% SDS PAGE
GTX121919 diluted at 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-EGFR antibody [N1-2], N-term used in Western Blot and Immunoprecipitation (WB IP). GTX121919

GTX121919 WB IP Image

Orthogonal

The data was published in the journal Nat Commun in 2014. PMID: 25366117

Anti-EGFR antibody [N1-2], N-term used in Western Blot and Immunoprecipitation (WB IP). GTX121919

GTX121919 WB IP Image

Orthogonal

The data was published in the journal Nat Commun in 2014. PMID: 25366117

Anti-EGFR antibody [N1-2], N-term used in Western Blot (WB). GTX121919

GTX121919 WB Image

KO/KD

The data was published in the journal Nat Commun in 2014. PMID: 25366117

Anti-EGFR antibody [N1-2], N-term used in Western Blot (WB). GTX121919

GTX121919 WB Image

The data was published in the journal PLoS One in 2014.PMID: 25180832

Anti-EGFR antibody [N1-2], N-term used in Western Blot and Immunoprecipitation (WB IP). GTX121919

GTX121919 WB IP Image

The data was published in the journal PLoS One in 2014.PMID: 25180832

Anti-EGFR antibody [N1-2], N-term used in Western Blot (WB). GTX121919

GTX121919 WB Image

The data was published in the journal PLoS One in 2014.PMID: 25180832

REFERENCE

Application Reference

Huang HN et al. Int J Mol Sci 2024; 25 (3) Leucine Zipper Downregulated in Cancer-1 Interacts with Clathrin Adaptors to Control Epidermal Growth Factor Receptor (EGFR) Internalization and Gefitinib Response in EGFR-Mutated Non-Small Cell Lung Cancer.
HN Huang et al. Int J Mol Sci 2024; 25 (3) : 1374 Leucine Zipper Downregulated in Cancer-1 Interacts with Clathrin Adaptors to Control Epidermal Growth Factor Receptor (EGFR) Internalization and Gefitinib Response in EGFR-Mutated Non-Small Cell Lung Cancer
Application : WB
Phan QT et al. PLoS Pathog 2023; 19 (8) : e1011579 Candida albicans stimulates formation of a multi-receptor complex that mediates epithelial cell invasion during oropharyngeal infection.
Nango H et al. PLoS One 2023; 18 (4) : e0284713 S-1-propenyl-L-cysteine suppresses lipopolysaccharide-induced expression of matrix metalloproteinase-1 through inhibition of tumor necrosis factor-α converting enzyme-epidermal growth factor receptor axis in human gingival fibroblasts.
Phan QT et al. bioRxiv 2023; Candida albicans stimulates the formation of a multi-receptor complex that mediates epithelial cell invasion during oropharyngeal infection.
Application : PLA
Show More +
Submit a Reference

REVIEW

EGFR antibody [N1-2], N-term Cat. No. GTX121919

Rating
( Average 4.5 based on 2 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Flow cytometry(FACS)
( Average 4 based on 1 users reviews)
Anti-EGFR antibody [N1-2], N-term used in Western Blot (WB). GTX121919
Date : Anonymous submitted on 08-Mar-2022
Score :
Application Tested : WB
Sample Species : Hu
Sample : HSC-3, OECM-1, and SAS cells
Amount used : 50μg
Blocking : 5% non-fat milk in TBST, 30°C, 1Hr
Primary Antibody : 1:1000, 4°C, 18Hr
Anti-EGFR antibody [N1-2], N-term used in Flow cytometry (FACS). GTX121919
Date : Anonymous submitted on 18-Jul-2013
Score :
Application Tested : FACS
Sample Species : Hu
Sample : MSP cells
Blocking : 0.2% FBS in PBS, 4°C, 0.17Hr
Primary Antibody : 1:250, 4°C, 1Hr
Submit a Review
Application Reference
Huang HN et al. Int J Mol Sci 2024; 25 (3) Leucine Zipper Downregulated in Cancer-1 Interacts with Clathrin Adaptors to Control Epidermal Growth Factor Receptor (EGFR) Internalization and Gefitinib Response in EGFR-Mutated Non-Small Cell Lung Cancer.
HN Huang et al. Int J Mol Sci 2024; 25 (3):1374 Leucine Zipper Downregulated in Cancer-1 Interacts with Clathrin Adaptors to Control Epidermal Growth Factor Receptor (EGFR) Internalization and Gefitinib Response in EGFR-Mutated Non-Small Cell Lung Cancer
Application : WB
Phan QT et al. PLoS Pathog 2023; 19 (8):e1011579 Candida albicans stimulates formation of a multi-receptor complex that mediates epithelial cell invasion during oropharyngeal infection.
Nango H et al. PLoS One 2023; 18 (4):e0284713 S-1-propenyl-L-cysteine suppresses lipopolysaccharide-induced expression of matrix metalloproteinase-1 through inhibition of tumor necrosis factor-α converting enzyme-epidermal growth factor receptor axis in human gingival fibroblasts.
Phan QT et al. bioRxiv 2023; Candida albicans stimulates the formation of a multi-receptor complex that mediates epithelial cell invasion during oropharyngeal infection.
Application : PLA
Seung-Hun Kim et al. Cancer Drug Resist 2021; 4 (4):866-880 The aqueous extract of brucea javanica reduces tumorigenicity of human lung cancer tumorspheres.
Application : WB
Reactivity : Human
Han-Lin Hsu et al. Int J Mol Sci 2021; 22 (18) Aryl Hydrocarbon Receptor Defect Attenuates Mitogen-Activated Signaling through Leucine-Rich Repeats and Immunoglobulin-like Domains 1 (LRIG1)-Dependent EGFR Degradation.
Reactivity : Mouse
Quynh T Phan et al. mBio 2021;:e0271621 The Globular C1q Receptor Is Required for Epidermal Growth Factor Receptor Signaling during Candida albicans Infection.
Application : PLA
Reactivity : Human
Yeh CH et al. Pharmaceutics 2021; 13 (9) Novel Pyropheophorbide Phosphatydic Acids Photosensitizer Combined EGFR siRNA Gene Therapy for Head and Neck Cancer Treatment.
Application : WB
Reactivity : Human
Jao TM et al. Cancer Lett 2021; 499:290-300 PCDH10 exerts tumor-suppressor functions through modulation of EGFR/AKT axis in colorectal cancer.
Application : WB
Reactivity : Human
Jia X et al. Mol Med Rep 2020; 21 (4):1779-1788 HER4 promotes the progression of colorectal cancer by promoting epithelial?mesenchymal transition.
Li J et al. J Ethnopharmacol 2020; 257:112796 Effective-component compatibility of Bufei Yishen formula II inhibits mucus hypersecretion of chronic obstructive pulmonary disease rats by regulating EGFR/PI3K/mTOR signaling.
Application : WB, IHC-P
Reactivity : Rat
Wang CH et al. Sci Rep 2020; 10 (1):2187 The novel application of cordycepin in maintaining stem cell pluripotency and increasing iPS cell generation efficiency.
Application : WB
Reactivity : Mouse
Xiaojing Jia et al. Molecular Medicine Reports 2020;:1779-1788 HER4 promotes the progression of colorectal cancer by promoting epithelial?mesenchymal transition.
Application : IHC-P
Reactivity : Human
Huang WT et al. Carbohydr Polym 2019; 203:256-264 A new type of gadodiamide-conjugated amphiphilic chitosan nanoparticle and its use for MR imaging with significantly enhanced contrastability.
Chen CF et al. Oncology Letters 2018;:6323-6330 synergistic inhibitory effects of * and curcumin on human *-resistant oral cancer car cells through intrinsic apoptotic process.
Application : WB
Reactivity : Human
Chang TC et al. Horm Cancer 2018; (Epub) enhancement by nano-diamino-tetrac of antiproliferative action of * on colorectal cancer cells: mediation by egfr sialylation and pi3k activation.
Application : ICC/IF
Reactivity : Human
Sasaki N et al. Stem Cell Res Ther 2017; 8 (1):81 Sialylation regulates myofibroblast differentiation of human skin fibroblasts.
Application : FACS
Reactivity : Human
Chen PH et al. Neuropharmacology 2016; (Epub) the chac1-inhibited notch3 pathway is involved in *-induced glioma cytotoxicity.
Application : WB
Reactivity : Human
Shostak K et al. Nat Commun 2014; 5:5232 NF-κB-induced KIAA1199 promotes survival through EGFR signalling.
Application : IP
Reactivity : Human
Jerman S et al. PLoS One 2014; 9 (9):e106330 OFD1 and Flotillins Are Integral Components of a Ciliary Signaling Protein Complex Organized by Polycystins in Renal Epithelia and Odontoblasts.
Application : ICC/IF
Reactivity : Human
Liu CH et al. Cell Transplant 2014; 23 (4):657-68 Salvianolic Acid B Maintained Stem Cell Pluripotency and Increased Proliferation Rate by Activating Jak2-Stat3 Combined With EGFR-Erk1/2 Pathways.
Application : WB
Reactivity : Mouse
Tsai YC et al. Eur J Cancer 2013; 49 (6):1458-66 Targeting epidermal growth factor receptor/human epidermal growth factor receptor 2 signalling pathway by a dual receptor tyrosine kinase inhibitor afatinib for radiosensitisation in murine bladder carcinoma.
Application : WB
Reactivity : Mouse
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169