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EGFR antibody [N1-2], N-term

Anti-EGFR antibody [N1-2], N-term used in IHC (Paraffin sections) (IHC-P). GTX121919
Anti-EGFR antibody [N1-2], N-term used in Western Blot (WB). GTX121919
Anti-EGFR antibody [N1-2], N-term used in IHC (Paraffin sections) (IHC-P). GTX121919
Anti-EGFR antibody [N1-2], N-term used in Western Blot (WB). GTX121919
Anti-EGFR antibody [N1-2], N-term used in Western Blot (WB). GTX121919
Anti-EGFR antibody [N1-2], N-term used in Western Blot (WB). GTX121919
Anti-EGFR antibody [N1-2], N-term used in Immunoprecipitation (IP). GTX121919
Anti-EGFR antibody [N1-2], N-term used in Western Blot (WB). GTX121919

Cat No. GTX121919

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, FACS, IP

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
FACS Assay dependent
IP 1:100-1:500
Not tested in other applications.

Calculated MW

134 kDa. ( Note )

Observed MW

180 kDa.

Positive Control

A431 , A549 , H1299 , HCT116 , Mouse brain , *MSP

Predict Reactivity

Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.04 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human EGFR. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10729577

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

epidermal growth factor receptor , ERBB , ERBB1 , HER1 , NISBD2 , PIG61 , mENA

Cellular Localization

Cell membrane; Single-pass type I membrane protein , Isoform 2: Secreted

Background

The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-EGFR antibody [N1-2], N-term used in IHC (Paraffin sections) (IHC-P). GTX121919

GTX121919 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using EGFR(GTX121919) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-EGFR antibody [N1-2], N-term used in Western Blot (WB). GTX121919

GTX121919 WB Image

Non-transfected (–) and transfected (+) A431 whole cell extracts (15 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with EGFR antibody [N1-2], N-term (GTX121919) diluted at 1:12000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-EGFR antibody [N1-2], N-term used in IHC (Paraffin sections) (IHC-P). GTX121919

GTX121919 IHC-P Image

EGFR antibody [N1-2], N-term detects EGFR protein at cell membrane in human non-small cell lung cancer by immunohistochemical analysis.
Sample: Two independent paraffin-embedded human non-small cell lung cancer.
EGFR antibody [N1-2], N-term (GTX121919) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-EGFR antibody [N1-2], N-term used in Western Blot (WB). GTX121919

GTX121919 WB Image

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membranes were blotted with EGFR antibody [N1-2], N-term (GTX121919) diluted at 1:500 and competitor's antibody (UM500070) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-EGFR antibody [N1-2], N-term used in Western Blot (WB). GTX121919

GTX121919 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with EGFR antibody [N1-2], N-term (GTX121919) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-EGFR antibody [N1-2], N-term used in Western Blot (WB). GTX121919

GTX121919 WB Image

Sample (50 μg of whole cell lysate)
A: Mouse brain
5% SDS PAGE
GTX121919 diluted at 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-EGFR antibody [N1-2], N-term used in Immunoprecipitation (IP). GTX121919

GTX121919 IP Image

EGFR protein was immunoprecipitated from A549 cell lysate using 5 ug of anti-EGFR antibody (GTX121919). The precipitated EGFR protein was detected by GTX121919 diluted at 1:500, and used EasyBlot anti-rabbit IgG (GTX221666-01) as secondary reagent.
A: A549 cell lysate.
B: IP by EGFR antibody.
7.5% SDS PAGE

Anti-EGFR antibody [N1-2], N-term used in Western Blot (WB). GTX121919

GTX121919 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with EGFR antibody [N1-2], N-term (GTX121919) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

EGFR antibody [N1-2], N-term Cat No. GTX121919

Rating ( Average 4.9 based on 7 users reviews)
Application
Western Blot(WB) ( Average 5 based on 6 users reviews)
Flow cytometry(FACS) ( Average 4 based on 1 users reviews)
Immunoprecipitation(IP) ( Average 5 based on 3 users reviews)
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : IP,WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2014.PMID: 25180832
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2014.PMID: 25180832
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2014.PMID: 25180832
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB,IP
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2014.PMID: 25366117
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB,IP
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2014.PMID: 25366117
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2014.PMID: 25366117
Date : Anonymous submitted on 18-Jul-2013
Score :
Application Tested : FACS
Sample Species : Hu
Sample : MSP cells
Blocking : 0.2% FBS in PBS, 4°C, 0.17Hr
Primary Antibody : 1:250, 4°C, 1Hr
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169