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EGFR antibody [N1-2], N-term

Anti-EGFR antibody [N1-2], N-term used in IHC (Paraffin sections) (IHC-P). GTX121919
Anti-EGFR antibody [N1-2], N-term used in Western Blot (WB). GTX121919
Anti-EGFR antibody [N1-2], N-term used in IHC (Paraffin sections) (IHC-P). GTX121919
Anti-EGFR antibody [N1-2], N-term used in Western Blot (WB). GTX121919
Anti-EGFR antibody [N1-2], N-term used in Western Blot (WB). GTX121919
Anti-EGFR antibody [N1-2], N-term used in Immunoprecipitation (IP). GTX121919
Anti-EGFR antibody [N1-2], N-term used in Western Blot (WB). GTX121919

Cat No. GTX121919

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, FACS, IP
Reactivity Human, Mouse
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
FACS Assay dependent
IP 1:100-1:500
Not tested in other applications.

Calculated MW

134 kDa. ( Note )

Observed MW

180 kDa.

Positive Control

A431 , A549 , H1299 , HCT116 , Mouse brain , *MSP

Predict Reactivity

Rat, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.04 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human EGFR. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

epidermal growth factor receptor,ERBB,ERBB1,HER1,NISBD2,PIG61,mENA

Cellular Localization

Cell membrane; Single-pass type I membrane protein , Isoform 2: Secreted

Background

The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-EGFR antibody [N1-2], N-term used in IHC (Paraffin sections) (IHC-P). GTX121919

GTX121919 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using EGFR(GTX121919) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-EGFR antibody [N1-2], N-term used in Western Blot (WB). GTX121919

GTX121919 WB Image

Non-transfected (–) and transfected (+) A431 whole cell extracts (15 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with EGFR antibody [N1-2], N-term (GTX121919) diluted at 1:12000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-EGFR antibody [N1-2], N-term used in IHC (Paraffin sections) (IHC-P). GTX121919

GTX121919 IHC-P Image

EGFR antibody [N1-2], N-term detects EGFR protein at cell membrane in human non-small cell lung cancer by immunohistochemical analysis.
Sample: Two independent paraffin-embedded human non-small cell lung cancer.
EGFR antibody [N1-2], N-term (GTX121919) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-EGFR antibody [N1-2], N-term used in Western Blot (WB). GTX121919

GTX121919 WB Image

Whole cell extract (30 µg) was separated by 5% SDS-PAGE, and the membranes were blotted with EGFR antibody [N1-2], N-term (GTX121919) diluted at 1:500 and competitor's antibody (UM500070) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-EGFR antibody [N1-2], N-term used in Western Blot (WB). GTX121919

GTX121919 WB Image

Sample (50 µg of whole cell lysate)
A: Mouse brain
5% SDS PAGE
GTX121919 diluted at 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-EGFR antibody [N1-2], N-term used in Immunoprecipitation (IP). GTX121919

GTX121919 IP Image

EGFR protein was immunoprecipitated from A549 cell lysate using 5 ug of anti-EGFR antibody (GTX121919). The precipitated EGFR protein was detected by GTX121919 diluted at 1:500, and used EasyBlot anti-rabbit IgG (GTX221666-01) as secondary reagent.
A: A549 cell lysate.
B: IP by EGFR antibody.
7.5% SDS PAGE

Anti-EGFR antibody [N1-2], N-term used in Western Blot (WB). GTX121919

GTX121919 WB Image

Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with EGFR antibody [N1-2], N-term (GTX121919) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
REVIEW

EGFR antibody [N1-2], N-term Cat No. GTX121919

Rating ( Average 4 based on 1 users reviews)
Application
Flow cytometry(FACS) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 18-Jul-2013
Score :
Application Tested : FACS
Sample Species : Hu
Sample : MSP cells
Blocking : 0.2% FBS in PBS, 4°C, 0.17Hr
Primary Antibody : 1:250, 4°C, 1Hr
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169