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EGFR antibody [N1], N-term

Anti-EGFR antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX100725
Anti-EGFR antibody [N1], N-term used in Western Blot (WB). GTX100725
Anti-EGFR antibody [N1], N-term used in Western Blot (WB). GTX100725
Anti-EGFR antibody [N1], N-term used in Western Blot (WB). GTX100725
Anti-EGFR antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX100725
Anti-EGFR antibody [N1], N-term used in Western Blot (WB). GTX100725

Cat No. GTX100725

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

134 kDa. ( Note )

Observed MW

65 kDa.

Positive Control

A431 , A549 , H1299 , HCT116

Predict Reactivity

Rat, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human EGFR. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950177

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

epidermal growth factor receptor , ERBB , ERBB1 , HER1 , NISBD2 , PIG61 , mENA

Cellular Localization

Cell membrane; Single-pass type I membrane protein , Isoform 2: Secreted

Background

The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-EGFR antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX100725

GTX100725 IHC-P Image

Immunohistochemical analysis of paraffin-embedded CA922 xenograft, using EGFR(GTX100725) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-EGFR antibody [N1], N-term used in Western Blot (WB). GTX100725

GTX100725 WB Image

Non-transfected (–) and transfected (+) A431 whole cell extracts (15 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with EGFR antibody [N1], N-term (GTX100725) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-EGFR antibody [N1], N-term used in Western Blot (WB). GTX100725

GTX100725 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with EGFR antibody [N1], N-term (GTX100725) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-EGFR antibody [N1], N-term used in Western Blot (WB). GTX100725

GTX100725 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EGFR antibody [N1], N-term (GTX100725) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-EGFR antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX100725

GTX100725 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using EGFR(GTX100725) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-EGFR antibody [N1], N-term used in Western Blot (WB). GTX100725

GTX100725 WB Image

EGFR antibody detects EGFR protein by western blot analysis. Whole cell extracts (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with EGFR antibody (GTX100725) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

There are currently no references for EGFR antibody [N1], N-term (GTX100725). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for EGFR antibody [N1], N-term (GTX100725). Be the first to share your experience with this product.
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 379
$ 169