GeneTex

  • Country / Location Selection

United States (US)

EGFR antibody [N1], N-term

KO/KD Orthogonal
Anti-EGFR antibody [N1], N-term used in Western Blot (WB). GTX100725
Anti-EGFR antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX100725
Anti-EGFR antibody [N1], N-term used in Western Blot (WB). GTX100725
Anti-EGFR antibody [N1], N-term used in Western Blot (WB). GTX100725
Anti-EGFR antibody [N1], N-term used in Western Blot (WB). GTX100725
Anti-EGFR antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX100725

Cat. No. GTX100725

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all EGFR products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

134 kDa. ( Note )

Observed MW

65 kDa.

Positive Control

A431 , A549 , H1299 , HCT116

Predict Reactivity

Mouse, Rat, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide encompassing a sequence within the Extracellular domain of human EGFR. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950177

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

epidermal growth factor receptor , ERBB , ERBB1 , HER1 , NISBD2 , PIG61 , mENA

Cellular Localization

Cell membrane; Single-pass type I membrane protein , Isoform 2: Secreted

Background

The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-EGFR antibody [N1], N-term used in Western Blot (WB). GTX100725

GTX100725 WB Image

KO/KD

Non-transfected (–) and transfected (+) A431 whole cell extracts (15 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with EGFR antibody [N1], N-term (GTX100725) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-EGFR antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX100725

GTX100725 IHC-P Image

Immunohistochemical analysis of paraffin-embedded CA922 xenograft, using EGFR(GTX100725) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-EGFR antibody [N1], N-term used in Western Blot (WB). GTX100725

GTX100725 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with EGFR antibody [N1], N-term (GTX100725) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-EGFR antibody [N1], N-term used in Western Blot (WB). GTX100725

GTX100725 WB Image

EGFR antibody detects EGFR protein by western blot analysis. Whole cell extracts (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with EGFR antibody (GTX100725) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-EGFR antibody [N1], N-term used in Western Blot (WB). GTX100725

GTX100725 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EGFR antibody [N1], N-term (GTX100725) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-EGFR antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX100725

GTX100725 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using EGFR(GTX100725) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

There are currently no references for EGFR antibody [N1], N-term (GTX100725). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for EGFR antibody [N1], N-term (GTX100725). Be the first to share your experience with this product.
Submit a Review
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169