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EGFR antibody

Anti-EGFR antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX35199
Anti-EGFR antibody used in IHC (Paraffin sections) (IHC-P). GTX35199
Anti-EGFR antibody used in IHC (Paraffin sections) (IHC-P). GTX35199
Anti-EGFR antibody used in Western Blot (WB). GTX35199
Anti-EGFR antibody used in IHC (Paraffin sections) (IHC-P). GTX35199
Anti-EGFR antibody used in Immunoprecipitation (IP). GTX35199

Cat. No. GTX35199

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all EGFR products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
IP 1:50 - 1:100
Not tested in other applications.

Calculated MW

134 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1021-1210 of human EGFR (NP_005219.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

epidermal growth factor receptor , ERBB , ERBB1 , HER1 , NISBD2 , PIG61 , mENA

Cellular Localization

Cell membrane,Endoplasmic reticulum membrane,Endosome,Endosome membrane,Nucleus

Background

The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq, Jun 2016]

Database

Research Area

DATA IMAGES

Anti-EGFR antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX35199

GTX35199 ICC/IF Image

ICC/IF analysis of A431 cells using GTX35199 EGFR antibody.
Orange : Primary antibody
Blue : DAPI
Dilution : 1:500

Anti-EGFR antibody used in IHC (Paraffin sections) (IHC-P). GTX35199

GTX35199 IHC-P Image

IHC-P analysis of mouse heart tissue using GTX35199 EGFR antibody.
Antigen retrieval : microwave antigen retrieval with 10mM PBS buffer (pH 7.2)
Dilution : 1:200

Anti-EGFR antibody used in IHC (Paraffin sections) (IHC-P). GTX35199

GTX35199 IHC-P Image

IHC-P analysis of rat liver tissue using GTX35199 EGFR antibody.
Antigen retrieval : microwave antigen retrieval with 10mM PBS buffer (pH 7.2)
Dilution : 1:200

Anti-EGFR antibody used in Western Blot (WB). GTX35199

GTX35199 WB Image

WB analysis of A431 whole cell lysate using GTX35199 EGFR antibody.
Dilution : 1:2000
Loading : 25μg per lane

Anti-EGFR antibody used in IHC (Paraffin sections) (IHC-P). GTX35199

GTX35199 IHC-P Image

IHC-P analysis of human placenta tissue using GTX35199 EGFR antibody.
Antigen retrieval : microwave antigen retrieval with 10mM PBS buffer (pH 7.2)
Dilution : 1:200

Anti-EGFR antibody used in Immunoprecipitation (IP). GTX35199

GTX35199 IP Image

IP analysis of HeLa cell lysate using GTX35199 EGFR antibody.
Antibody amount : 3μg / 200μg lysate
Dilution : 1:1000

REFERENCE

Application Reference

S Mazzucchelli et al. Cancer Cell Int 2024; 24 (1) : 220 Breast cancer patient-derived organoids for the investigation of patient-specific tumour evolution
Application : ICC/IF
S Mazzucchelli et al. Cancer Cell Int 2024; 24 (1) : 220 Breast cancer patient-derived organoids for the investigation of patient-specific tumour evolution
Application : ICC/IF
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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399