GeneTex
United States (US)

EGFR antibody

KO/KD
GTX35199_20180822_IHC-P_1_18121410_875.jpg
GTX35199_20180822_ICCIF_18121410_773.jpg
GTX35199_20180822_IHC-P_2_18121410_354.jpg
GTX35199_20180822_ICCIF_2_18121410_464.jpg
GTX35199_20180822_ICCIF_1_18121410_666.jpg
GTX35199_20180822_IHC-P_3_18121410_844.jpg
GTX35199_20180822_IHC-P_18121410_545.jpg
GTX35199_20180822_IP_18121410_501.jpg
GTX35199_20180822_WB_1_18121410_409.jpg
GTX35199_20180822_WB_18121410_387.jpg

Cat No. GTX35199

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP, IHC
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
IP 1:50 - 1:100
IHC Assay dependent
Not tested in other applications.

Calculated MW

134 kDa kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 50% glycerol, 0.02% sodium azide

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1021-1210 of human EGFR (NP_005219.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

HER1 Antibody , ERBB1 Antibody , mENA Antibody , PIG61 Antibody , ERBB Antibody , epidermal growth factor receptor Antibody

Background

The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. Multiple alternatively spliced transcript variants that encode different protein isoforms have been found for this gene. [provided by RefSeq, Jul 2010]
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DATA IMAGES
GTX35199_20180822_IHC-P_1_18121410_875.jpg

GTX35199 IHC-P Image

KO/KD

IHC-P analysis of human prostate tissue using GTX35199 EGFR antibody.
Dilution : 1:100
GTX35199_20180822_ICCIF_18121410_773.jpg

GTX35199 ICC/IF Image

KO/KD

ICC/IF ananlysis of HeLa cells using GTX35199 EGFR antibody.
Dilution : 1:100
Blue : DAPI
GTX35199_20180822_IHC-P_2_18121410_354.jpg

GTX35199 IHC-P Image

KO/KD

IHC-P analysis of mouse brain tissue using GTX35199 EGFR antibody.
Dilution : 1:100
GTX35199_20180822_ICCIF_2_18121410_464.jpg

GTX35199 ICC/IF Image

KO/KD

ICC/IF ananlysis of PC12 cells using GTX35199 EGFR antibody.
Dilution : 1:100
Blue : DAPI
GTX35199_20180822_ICCIF_1_18121410_666.jpg

GTX35199 ICC/IF Image

KO/KD

ICC/IF ananlysis of NIH/3T3 cells using GTX35199 EGFR antibody.
Dilution : 1:100
Blue : DAPI
GTX35199_20180822_IHC-P_3_18121410_844.jpg

GTX35199 IHC-P Image

KO/KD

IHC-P analysis of mouse heart tissue using GTX35199 EGFR antibody.
Dilution : 1:100
GTX35199_20180822_IHC-P_18121410_545.jpg

GTX35199 IHC-P Image

KO/KD

IHC-P analysis of rat lung tissue using GTX35199 EGFR antibody.
Dilution : 1:100
GTX35199_20180822_IP_18121410_501.jpg

GTX35199 IP Image

KO/KD

IP analysis of Hela cell lysate using GTX35199 EGFR antibody.
IP reaction : 3μg antibody / 200μg cell lysate
Dilution : 1:1000
GTX35199_20180822_WB_1_18121410_409.jpg

GTX35199 WB Image

KO/KD

WB analysis of wild type and EGFR knockout cell lysate using GTX35199 EGFR antibody.
Dilution : 1:500
Loading : 25μg per lane
GTX35199_20180822_WB_18121410_387.jpg

GTX35199 WB Image

KO/KD

WB analysis of various samples using GTX35199 EGFR antibody.
Dilution : 1:1000
Loading : 25μg per lane
Package List Price ($)
$ 329