APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:500 - 1:2000 |
| 1:50 - 1:200 |
| 1:50 - 1:200 |
| 1:50 - 1:100 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS pH7.3, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1021-1210 of human EGFR (NP_005219.2).
Purification
Purified by affinity chromatography
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
epidermal growth factor receptor , ERBB , ERBB1 , HER1 , NISBD2 , PIG61 , mENA
Cellular Localization
Cell membrane,Endoplasmic reticulum membrane,Endosome,Endosome membrane,Nucleus
Background
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq, Jun 2016]
Database
Research Area
DATA IMAGES
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GTX35199 IP Image
IP analysis of HeLa cell lysate using GTX35199 EGFR antibody.
Antibody amount : 3μg / 200μg lysate
Dilution : 1:1000
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GTX35199 ICC/IF Image
ICC/IF analysis of NIH/3T3 cells using GTX35199 EGFR antibody.
Blue : DAPI
Dilution : 1:100
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GTX35199 ICC/IF Image
ICC/IF analysis of PC-12 cells using GTX35199 EGFR antibody.
Blue : DAPI
Dilution : 1:100
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GTX35199 WB Image
WB analysis of normal (control) and knockout (KO) HeLa cell lysate using GTX35199 EGFR antibody.
Dilution : 1:3000
Loading : 25μg per lane
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GTX35199 WB Image
WB analysis of OVCAR3 cell lysate using GTX35199 EGFR antibody.
Dilution : 1:3000
Loading : 25μg per lane
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GTX35199 ICC/IF Image
ICC/IF analysis of HeLa cells using GTX35199 EGFR antibody.
Blue : DAPI
Dilution : 1:100
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GTX35199 IHC-P Image
IHC-P analysis of human prostate tissue using GTX35199 EGFR antibody.
Dilution : 1:100
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GTX35199 IHC-P Image
IHC-P analysis of mouse brain tissue using GTX35199 EGFR antibody.
Dilution : 1:100
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REFERENCE
There are currently no references for EGFR antibody (GTX35199). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for EGFR antibody (GTX35199). Be the first to share your experience with this product.
