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EGFR antibody

Anti-EGFR antibody used in Immunoprecipitation (IP). GTX35199
Anti-EGFR antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX35199
Anti-EGFR antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX35199
Anti-EGFR antibody used in Western Blot (WB). GTX35199
Anti-EGFR antibody used in Western Blot (WB). GTX35199
Anti-EGFR antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX35199
Anti-EGFR antibody used in IHC (Paraffin sections) (IHC-P). GTX35199
Anti-EGFR antibody used in IHC (Paraffin sections) (IHC-P). GTX35199

Cat No. GTX35199

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IP

Reactivity

Human, Mouse, Rat
Package
100 μl ($379)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
IP 1:50 - 1:100
Not tested in other applications.

Calculated MW

134 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1021-1210 of human EGFR (NP_005219.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

epidermal growth factor receptor , ERBB , ERBB1 , HER1 , NISBD2 , PIG61 , mENA

Cellular Localization

Cell membrane,Endoplasmic reticulum membrane,Endosome,Endosome membrane,Nucleus

Background

The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq, Jun 2016]

Database

Research Area

DATA IMAGES

Anti-EGFR antibody used in Immunoprecipitation (IP). GTX35199

GTX35199 IP Image

IP analysis of HeLa cell lysate using GTX35199 EGFR antibody.
Antibody amount : 3μg / 200μg lysate
Dilution : 1:1000

Anti-EGFR antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX35199

GTX35199 ICC/IF Image

ICC/IF analysis of NIH/3T3 cells using GTX35199 EGFR antibody.
Blue : DAPI
Dilution : 1:100

Anti-EGFR antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX35199

GTX35199 ICC/IF Image

ICC/IF analysis of PC-12 cells using GTX35199 EGFR antibody.
Blue : DAPI
Dilution : 1:100

Anti-EGFR antibody used in Western Blot (WB). GTX35199

GTX35199 WB Image

WB analysis of normal (control) and knockout (KO) HeLa cell lysate using GTX35199 EGFR antibody.
Dilution : 1:3000
Loading : 25μg per lane

Anti-EGFR antibody used in Western Blot (WB). GTX35199

GTX35199 WB Image

WB analysis of OVCAR3 cell lysate using GTX35199 EGFR antibody.
Dilution : 1:3000
Loading : 25μg per lane

Anti-EGFR antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX35199

GTX35199 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX35199 EGFR antibody.
Blue : DAPI
Dilution : 1:100

Anti-EGFR antibody used in IHC (Paraffin sections) (IHC-P). GTX35199

GTX35199 IHC-P Image

IHC-P analysis of human prostate tissue using GTX35199 EGFR antibody.
Dilution : 1:100

Anti-EGFR antibody used in IHC (Paraffin sections) (IHC-P). GTX35199

GTX35199 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX35199 EGFR antibody.
Dilution : 1:100

REFERENCE

There are currently no references for EGFR antibody (GTX35199). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for EGFR antibody (GTX35199). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 379