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EGFR antibody

Orthogonal
Anti-EGFR antibody used in IHC (Paraffin sections) (IHC-P). GTX50426
Anti-EGFR antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50426
Anti-EGFR antibody used in Western Blot (WB). GTX50426

Cat. No. GTX50426

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399)
See all EGFR products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
ICC/IF 1:100-1:200
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

134 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around aa.1068~1072 (R-Y-S-S-D) derived from human EGFR.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

epidermal growth factor receptor , ERBB , ERBB1 , HER1 , NISBD2 , PIG61 , mENA

Cellular Localization

Cell membrane,Endoplasmic reticulum membrane,Endosome,Endosome membrane,Nucleus

Background

The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq, Jun 2016]

Database

Research Area

DATA IMAGES

Anti-EGFR antibody used in IHC (Paraffin sections) (IHC-P). GTX50426

GTX50426 IHC-P Image

Orthogonal

IHC-P analysis of human breast carcinoma tissue using GTX50426 EGFR antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

Anti-EGFR antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50426

GTX50426 ICC/IF Image

Orthogonal

ICC/IF analysis of methanol-fixed MCF cells using GTX50426 EGFR antibody.

Anti-EGFR antibody used in Western Blot (WB). GTX50426

GTX50426 WB Image

Orthogonal

WB analysis of extracts from HUVEC and MDA cells using GTX50426 EGFR antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399