APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1/500 - 1/2000 |
| 1/200 - 1/1000 |
| 1/200 - 1/400 |
| 1/10000 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Purified recombinant fragment of human EGFR mutant (AA: 693-893) expressed in E. Coli.
Purification
Protein G Purified
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
epidermal growth factor receptor , ERBB , ERBB1 , HER1 , NISBD2 , PIG61 , mENA
Cellular Localization
Cell membrane,Endoplasmic reticulum membrane,Endosome,Endosome membrane,Nucleus
Background
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. Multiple alternatively spliced transcript variants that encode different protein isoforms have been found for this gene. [provided by RefSeq, Jul 2010]
Database
Research Area
DATA IMAGES
![Anti-EGFR (mutant) antibody [5G9B5] used in IHC (Paraffin sections) (IHC-P). GTX60766](/templates/default/js/lightbox/images/loading.gif "Anti-EGFR (mutant) antibody [5G9B5] used in IHC (Paraffin sections) (IHC-P). GTX60766")
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GTX60766 IHC-P Image
IHC-P analysis of cervical cancer tissue using GTX60766 EGFR (mutant) antibody [5G9B5].
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GTX60766 ELISA Image
ELISA analysis of antigen using GTX60766 EGFR (mutant) antibody [5G9B5].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng
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GTX60766 FACS Image
FACS analysis of HepG2 cells using GTX60766 EGFR (mutant) antibody [5G9B5].
Green : EGFR (mutant)
Purple : negative control
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GTX60766 IHC-P Image
IHC-P analysis of rectum cancer tissue using GTX60766 EGFR (mutant) antibody [5G9B5].
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GTX60766 WB Image
WB analysis of HEK293 (1) and EGFR mutant (AA: 693-893)-hIgGFc transfected HEK293 (2) cell lysate using GTX60766 EGFR (mutant) antibody [5G9B5].
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GTX60766 WB Image
WB analysis of SPC-A-1 (1), A549 (2), HepG2 (3) and MCF-7 (4) cell lysate using GTX60766 EGFR (mutant) antibody [5G9B5].
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REFERENCE
There are currently no references for EGFR (mutant) antibody [5G9B5] (GTX60766). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for EGFR (mutant) antibody [5G9B5] (GTX60766). Be the first to share your experience with this product.
![Anti-EGFR (mutant) antibody [5G9B5] used in IHC (Paraffin sections) (IHC-P). GTX60766](/upload/website/prouct_img/small/GTX60766/GTX60766_20170912_IHC-P_18121411_633.jpg "Anti-EGFR (mutant) antibody [5G9B5] used in IHC (Paraffin sections) (IHC-P). GTX60766")
![Anti-EGFR (mutant) antibody [5G9B5] used in ELISA (ELISA). GTX60766](/upload/website/prouct_img/small/GTX60766/GTX60766_20170912_ELISA_18121411_391.jpg "Anti-EGFR (mutant) antibody [5G9B5] used in ELISA (ELISA). GTX60766")
![Anti-EGFR (mutant) antibody [5G9B5] used in Flow cytometry (FACS). GTX60766](/upload/website/prouct_img/small/GTX60766/GTX60766_20170912_FACS_18121411_424.jpg "Anti-EGFR (mutant) antibody [5G9B5] used in Flow cytometry (FACS). GTX60766")
![Anti-EGFR (mutant) antibody [5G9B5] used in IHC (Paraffin sections) (IHC-P). GTX60766](/upload/website/prouct_img/small/GTX60766/GTX60766_20170912_IHC-P_1_18121411_201.jpg "Anti-EGFR (mutant) antibody [5G9B5] used in IHC (Paraffin sections) (IHC-P). GTX60766")
![Anti-EGFR (mutant) antibody [5G9B5] used in Western Blot (WB). GTX60766](/upload/website/prouct_img/small/GTX60766/GTX60766_20170912_WB_18121411_845.jpg "Anti-EGFR (mutant) antibody [5G9B5] used in Western Blot (WB). GTX60766")
![Anti-EGFR (mutant) antibody [5G9B5] used in Western Blot (WB). GTX60766](/upload/website/prouct_img/small/GTX60766/GTX60766_20170912_WB_1_18121411_924.jpg "Anti-EGFR (mutant) antibody [5G9B5] used in Western Blot (WB). GTX60766")
![Anti-EGFR (mutant) antibody [5G9B5] used in IHC (Paraffin sections) (IHC-P). GTX60766](/upload/website/prouct_img/normal/GTX60766/GTX60766_20170912_IHC-P_18121411_633.jpg "Anti-EGFR (mutant) antibody [5G9B5] used in IHC (Paraffin sections) (IHC-P). GTX60766")
![Anti-EGFR (mutant) antibody [5G9B5] used in ELISA (ELISA). GTX60766](/upload/website/prouct_img/normal/GTX60766/GTX60766_20170912_ELISA_18121411_391.jpg "Anti-EGFR (mutant) antibody [5G9B5] used in ELISA (ELISA). GTX60766")
![Anti-EGFR (mutant) antibody [5G9B5] used in Flow cytometry (FACS). GTX60766](/upload/website/prouct_img/normal/GTX60766/GTX60766_20170912_FACS_18121411_424.jpg "Anti-EGFR (mutant) antibody [5G9B5] used in Flow cytometry (FACS). GTX60766")
![Anti-EGFR (mutant) antibody [5G9B5] used in IHC (Paraffin sections) (IHC-P). GTX60766](/upload/website/prouct_img/normal/GTX60766/GTX60766_20170912_IHC-P_1_18121411_201.jpg "Anti-EGFR (mutant) antibody [5G9B5] used in IHC (Paraffin sections) (IHC-P). GTX60766")
![Anti-EGFR (mutant) antibody [5G9B5] used in Western Blot (WB). GTX60766](/upload/website/prouct_img/normal/GTX60766/GTX60766_20170912_WB_18121411_845.jpg "Anti-EGFR (mutant) antibody [5G9B5] used in Western Blot (WB). GTX60766")
![Anti-EGFR (mutant) antibody [5G9B5] used in Western Blot (WB). GTX60766](/upload/website/prouct_img/normal/GTX60766/GTX60766_20170912_WB_1_18121411_924.jpg "Anti-EGFR (mutant) antibody [5G9B5] used in Western Blot (WB). GTX60766")