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EGFR (phospho Thr654) antibody

Anti-EGFR (phospho Thr654) antibody used in Western Blot (WB). GTX32321

Cat. No. GTX32321

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all EGFR products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:1000
Not tested in other applications.

Calculated MW

134 kDa. ( Note )

Product Note

The full-length EGFR includes the 24 aa signal peptide that is cleaved during EGFR maturation. The Thr654 of mature EGFR is equivalent to Thr678 of uncleaved EGFR.

PROPERTIES

Form

Liquid

Buffer

0.42% Potassium Phosphate, 0.87% NaCl, 30% Glycerol

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human EGFR (pT654). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_2887651

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

epidermal growth factor receptor , ERBB , ERBB1 , HER1 , NISBD2 , PIG61 , mENA

Cellular Localization

Cell membrane,Endoplasmic reticulum membrane,Endosome,Endosome membrane,Nucleus

Background

The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq, Jun 2016]

Database

Research Area

DATA IMAGES

Anti-EGFR (phospho Thr654) antibody used in Western Blot (WB). GTX32321

GTX32321 WB Image

WB analysis of EGF-treated A431 (A), EGF-treated NIH3T3 (B), EGF-treated PC12 (C) whole cell lysates using GTX32321 EGFR (phospho Thr654) antibody.

REFERENCE

Application Reference

Dilday T et al. Cell Chem Biol 2024; 31 (5) : 989-999.e7 Identification and characterization of a potent and selective HUNK inhibitor for treatment of HER2+ breast cancer.
Williams CB et al. Oncogene 2019; HUNK phosphorylates EGFR to regulate breast cancer metastasis.
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SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399