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United States (US)

EGFR (phospho Thr654) antibody

Anti-EGFR (phospho Thr654) antibody used in Western Blot (WB). GTX32321

Cat No. GTX32321

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:1000
Not tested in other applications.

Calculated MW

134 kDa. ( Note )

Specificity/Sensitivity

The full-length EGFR includes the 24 aa signal peptide that is cleaved during EGFR maturation. The Thr654 of mature EGFR is equivalent to Thr678 of uncleaved EGFR.
PROPERTIES

Form

Liquid

Buffer

0.42% Potassium phosphate, 0.87% Sodium chloride pH 7.3, 30% glycerol, 0.01% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20º C or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of EGFR. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Epidermal Growth Factor Receptor,Erbb,Erbb1,Her1,Nisbd2,Pig61,Mena,Egfr

Cellular Localization

Cell membrane,Endoplasmic reticulum membrane,Endosome,Endosome membrane,Golgi apparatus membrane,Nucleus,Nucleus membrane,Secreted

Background

The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq, Jun 2016]

Database

Research Area

DATA IMAGES
Anti-EGFR (phospho Thr654) antibody used in Western Blot (WB). GTX32321

GTX32321 WB Image

WB analysis of EGF-treated A431 (A), EGF-treated NIH3T3 (B), EGF-treated PC12 (C) whole cell lysates using GTX32321 EGFR (phospho Thr654) antibody.

Package List Price ($)
$ 319