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EGFR (phospho Thr654) antibody

Anti-EGFR (phospho Thr654) antibody used in IHC (Paraffin sections) (IHC-P). GTX50233
Anti-EGFR (phospho Thr654) antibody used in Western Blot (WB). GTX50233

Cat. No. GTX50233

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Rat
Package
100 μl ($399)
See all EGFR products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

134 kDa. ( Note )

Product Note

The full-length EGFR includes the 24 aa signal peptide that is cleaved during EGFR maturation. The Thr654 of mature EGFR is equivalent to Thr678 of uncleaved EGFR.

Predict Reactivity

Mouse(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of threonine 654 (K-R-T(p)-L-R) derived from human EGFR.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

epidermal growth factor receptor , ERBB , ERBB1 , HER1 , NISBD2 , PIG61 , mENA

Cellular Localization

Cell membrane,Endoplasmic reticulum membrane,Endosome,Endosome membrane,Nucleus

Background

The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq, Jun 2016]

Database

Research Area

DATA IMAGES

Anti-EGFR (phospho Thr654) antibody used in IHC (Paraffin sections) (IHC-P). GTX50233

GTX50233 IHC-P Image

IHC-P analysis of rat colorectal tissue using GTX50233 EGFR (phospho Thr654) antibody.

Anti-EGFR (phospho Thr654) antibody used in Western Blot (WB). GTX50233

GTX50233 WB Image

WB analysis of extracts from MDA cells untreated or treated with EGF using GTX50233 EGFR (phospho Thr654) antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399