*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
A431 (Starvation for 16 hr and 100 ng/ml EGF treatment for 5 min)
The full-length EGFR includes the 24 aa signal peptide that is cleaved during EGFR maturation. The Thr669 of mature EGFR is equivalent to Thr693 of uncleaved EGFR.
Mouse, Rat, Chicken, Pig(>80% identity)
1XPBS (pH7), 1% BSA, 20% Glycerol
0.025% ProClin 300
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
0.3 mg/ml (Please refer to the vial label for the specific concentration.)
Carrier-protein conjugated synthetic peptide surrounding phospho Thr669 of human EGFR. The exact sequence is proprietary.
Affinity purified by Protein A.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
epidermal growth factor receptor , ERBB , ERBB1 , HER1 , NISBD2 , PIG61 , mENA
Cell membrane; Single-pass type I membrane protein , Isoform 2: Secreted
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq]
GTX133596 WB Image
Untreated (–) and treated (+) A431 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with EGFR (phospho Thr669) antibody (GTX133596) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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