GeneTex
United States (US)

EGFR (phospho Thr669) antibody

Anti-EGFR (phospho Thr669) antibody used in IHC (Paraffin sections) (IHC-P). GTX32385
Anti-EGFR (phospho Thr669) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32385
Anti-EGFR (phospho Thr669) antibody used in Western Blot (WB). GTX32385

Cat No. GTX32385

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:1000
ICC/IF 1:100 - 1:500
IHC-P 1:100 - 1:200
Not tested in other applications.

Calculated MW

134 kDa. ( Note )

Specificity/Sensitivity

The full-length EGFR includes the 24 aa signal peptide that is cleaved during EGFR maturation. The Thr669 of mature EGFR is equivalent to Thr693 of uncleaved EGFR.
PROPERTIES

Form

Liquid

Buffer

0.42% Potassium phosphate, 0.87% Sodium chloride pH 7.3, 30% glycerol, 0.01% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20º C or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of EGFR. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Epidermal Growth Factor Receptor,Erbb,Erbb1,Her1,Nisbd2,Pig61,Mena,Egfr

Cellular Localization

Cell membrane,Endoplasmic reticulum membrane,Endosome,Endosome membrane,Golgi apparatus membrane,Nucleus,Nucleus membrane,Secreted

Background

The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq, Jun 2016]

Database

Research Area

DATA IMAGES
Anti-EGFR (phospho Thr669) antibody used in IHC (Paraffin sections) (IHC-P). GTX32385

GTX32385 IHC-P Image

IHC-P analysis of formalin fixed human breast cancer tissue section using GTX32385 EGFR (phospho Thr669) antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)

Anti-EGFR (phospho Thr669) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32385

GTX32385 ICC/IF Image

ICC/IF analysis of formalin-fixed HEK293T cells using GTX32385 EGFR (phospho Thr669) antibody.
Red : Primary antibody
Blue : DAPI
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes

Anti-EGFR (phospho Thr669) antibody used in Western Blot (WB). GTX32385

GTX32385 WB Image

WB analysis of UV-treated HEK293T (A) whole cell lysates using GTX32385 EGFR (phospho Thr669) antibody.

Package List Price ($)
$ 319