*Optimal dilutions/concentrations should be determined by the researcher.
|1:500 - 1:1000
|1:100 - 1:500
|1:100 - 1:200
Not tested in other applications.
The full-length EGFR includes the 24 aa signal peptide that is cleaved during EGFR maturation. The Thr669 of mature EGFR is equivalent to Thr693 of uncleaved EGFR.
0.42% Potassium phosphate, 0.87% Sodium chloride pH 7.3, 30% glycerol, 0.01% sodium azide
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20º C or below. Avoid multiple freeze-thaw cycles.
KLH-conjugated synthetic peptide encompassing a sequence within the center region of EGFR. The exact sequence is proprietary.
Purified by antigen-affinity chromatography
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Epidermal Growth Factor Receptor,Erbb,Erbb1,Her1,Nisbd2,Pig61,Mena,Egfr
Cell membrane,Endoplasmic reticulum membrane,Endosome,Endosome membrane,Golgi apparatus membrane,Nucleus,Nucleus membrane,Secreted
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq, Jun 2016]
GTX32385 IHC-P Image
IHC-P analysis of formalin fixed human breast cancer tissue section using GTX32385 EGFR (phospho Thr669) antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)
GTX32385 ICC/IF Image
ICC/IF analysis of formalin-fixed HEK293T cells using GTX32385 EGFR (phospho Thr669) antibody.
Red : Primary antibody
Blue : DAPI
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes
GTX32385 WB Image
WB analysis of UV-treated HEK293T (A) whole cell lysates using GTX32385 EGFR (phospho Thr669) antibody.