*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
A431 (Starvation for 16 hr and then 100 ng/ml EGF treatment for 5 min)
The full-length EGFR includes the 24 aa signal peptide that is cleaved during EGFR maturation. The Tyr1045 of mature EGFR is equivalent to Tyr1069 of uncleaved EGFR.
Mouse, Rat, Pig(>80% identity)
1XPBS (pH7), 1% BSA, 20% Glycerol
0.025% ProClin 300
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
0.13 mg/ml (Please refer to the vial label for the specific concentration.)
Carrier-protein conjugated synthetic peptide surrounding phospho Tyr1045 of human EGFR. The exact sequence is proprietary.
Purified by antigen-affinity chromatography.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
epidermal growth factor receptor , ERBB , ERBB1 , HER1 , NISBD2 , PIG61 , mENA
Cell membrane; Single-pass type I membrane protein , Isoform 2: Secreted
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq]
GTX133431 IHC-P Image
EGFR (phospho Tyr1045) antibody detects EGFR (phospho Tyr1045) protein at cell membrane in human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
EGFR (phospho Tyr1045) antibody (GTX133431) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX133431 WB Image
Untreated (–) and treated (+) A431 whole cell extract (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EGFR (phospho Tyr1045) antibody (GTX133431) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX133431 ICC/IF Image
EGFR (phospho Tyr1045) antibody detects EGFR (phospho Tyr1045) protein at cell membrane by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: EGFR (phospho Tyr1045) protein stained by EGFR (phospho Tyr1045) antibody (GTX133431) diluted at 1:500.
Blue: Hoechst 33342 staining.
There are currently no references for EGFR (phospho Tyr1045) antibody (GTX133431). Be the first to share your publications with this product.
There are currently no reviews for EGFR (phospho Tyr1045) antibody (GTX133431). Be the first to share your experience with this product.