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EGFR (phospho Tyr1045) antibody

Anti-EGFR (phospho Tyr1045) antibody used in IHC (Paraffin sections) (IHC-P). GTX133431
Anti-EGFR (phospho Tyr1045) antibody used in Western Blot (WB). GTX133431
Anti-EGFR (phospho Tyr1045) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133431

Cat No. GTX133431

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

134 kDa. ( Note )

Positive Control

A431 (Starvation for 16 hr and then 100 ng/ml EGF treatment for 5 min)

Product Note

The full-length EGFR includes the 24 aa signal peptide that is cleaved during EGFR maturation. The Tyr1045 of mature EGFR is equivalent to Tyr1069 of uncleaved EGFR.

Predict Reactivity

Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.13 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Tyr1045 of human EGFR. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886980

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

epidermal growth factor receptor , ERBB , ERBB1 , HER1 , NISBD2 , PIG61 , mENA

Cellular Localization

Cell membrane; Single-pass type I membrane protein , Isoform 2: Secreted

Background

The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-EGFR (phospho Tyr1045) antibody used in IHC (Paraffin sections) (IHC-P). GTX133431

GTX133431 IHC-P Image

EGFR (phospho Tyr1045) antibody detects EGFR (phospho Tyr1045) protein at cell membrane in human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
EGFR (phospho Tyr1045) antibody (GTX133431) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-EGFR (phospho Tyr1045) antibody used in Western Blot (WB). GTX133431

GTX133431 WB Image

Untreated (–) and treated (+) A431 whole cell extract (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EGFR (phospho Tyr1045) antibody (GTX133431) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-EGFR (phospho Tyr1045) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133431

GTX133431 ICC/IF Image

EGFR (phospho Tyr1045) antibody detects EGFR (phospho Tyr1045) protein at cell membrane by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: EGFR (phospho Tyr1045) protein stained by EGFR (phospho Tyr1045) antibody (GTX133431) diluted at 1:500.
Blue: Hoechst 33342 staining.

REFERENCE

There are currently no references for EGFR (phospho Tyr1045) antibody (GTX133431). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for EGFR (phospho Tyr1045) antibody (GTX133431). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 379
$ 169