Untreated (–) and treated (+) A431 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with EGFR (phospho Tyr1068) antibody [GT1196] (GTX00958) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

WB analysis of A431 cell extracts with/without EGF (100 ng/ml, 37ºC, 30 mins) treatment using GTX00958 EGFR (phospho Tyr1068) antibody [GT1196].
Dilution : 1:1000
Loading : 25 μg