APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500 - 1:2000 |
Not tested in other applications.
Calculated MW
Product Note
The full-length EGFR includes the 24 aa signal peptide that is cleaved during EGFR maturation. The Tyr1068 of mature EGFR is equivalent to Tyr1092 of uncleaved EGFR.
PROPERTIES
Form
Liquid
Buffer
PBS, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
A synthesized peptide derived from human Phospho-EGFR (Y1068).
Purification
Purified by affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
EGFR,ERBB,ERBB1,HER1,NISBD2,PIG61,epidermal growth factor receptor,mENA,EGF Receptor
Cellular Localization
Cell membrane,Endoplasmic reticulum membrane,Golgi apparatus membrane,Nucleus membrane,Endosome,Endosome membrane,Nucleus,Secreted
Background
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq, Jun 2016]
Database
Research Area
DATA IMAGES
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GTX00958 WB Image
Untreated (–) and treated (+) A431 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with EGFR (phospho Tyr1068) antibody [GT1196] (GTX00958) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX00958 WB Image
WB analysis of A431 cell extracts with/without EGF (100 ng/ml, 37ºC, 30 mins) treatment using GTX00958 EGFR (phospho Tyr1068) antibody [GT1196]. Dilution : 1:1000 Loading : 25 μg
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REFERENCE
There are currently no references for EGFR (phospho Tyr1068) antibody [GT1196] (GTX00958). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for EGFR (phospho Tyr1068) antibody [GT1196] (GTX00958). Be the first to share your experience with this product.