EGFR (phospho Tyr1068) antibody

Cat. No. GTX25644

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC
Reactivity	Human, Mouse, Rat, Primate
Package	50 μl ($399)

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Datasheet
Document

View product citations for antibody GTX25644 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:1,000
ICC/IF	1:100
IHC	Assay dependent

Not tested in other applications.

Calculated MW

134 kDa. ( Note )

Product Note

The full-length EGFR includes the 24 aa signal peptide that is cleaved during EGFR maturation. The Tyr1068 of mature EGFR is equivalent to Tyr1092 of uncleaved EGFR.

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA, 50% Glycerol

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human EGFR that contains tyrosine 1068. The sequence is conserved in rat.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

epidermal growth factor receptor , ERBB , ERBB1 , HER1 , NISBD2 , PIG61 , mENA

Cellular Localization

Cell membrane,Endoplasmic reticulum membrane,Endosome,Endosome membrane,Nucleus

Background

The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq, Jun 2016]

Database

Gene ID: 1956 EGFR
UniProt: P00533 EGFR

Research Area

DATA IMAGES

GTX25644 ICC/IF Image

ICC/IF analysis of A431 cells treated with 200 ng/ml of EGF for 10 minutes using GTX25644 EGFR (phospho Tyr1068) antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 for 10 minutes

GTX25644 WB Image

WB analysis of membrane enriched extracts (30 ug lysate) of A431 (Lane 1) treated with EGF (200ng/ml for 10 minutes) (Lane 2), A431 treated with Gefitinib followed by EGF (1uM for 16 hours, 200ng/ml for 10 minutes) (Lane 3), A431 treated with Afatinib followed by EGF (0.5 uM for 6 hours, 200ng/ml for 10 minutes) (Lane 4), A549 (Lane 5) treated with EGF (200ng/ml for 10 minutes) (Lane 6), and A549 treated with Afatinib followed by EGF (0.5 uM for 6 hours, 200ng/ml for 10 minutes) (Lane 7) using GTX25644 EGFR (phospho Tyr1068) antibody.
Dilution : 1:1000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf

Package

List Price ($)

50 μl

$ 399

Qty