WB analysis of membrane enriched extracts (30 ug lysate) of A431 (Lane 1) treated with EGF (200ng/ml for 10 minutes) (Lane 2), A431 treated with Gefitinib followed by EGF (1uM for 16 hours, 200ng/ml for 10 minutes) (Lane 3), A431 treated with Afatinib followed by EGF (0.5 uM for 6 hours, 200ng/ml for 10 minutes) (Lane 4), A549 (Lane 5) treated with EGF (200ng/ml for 10 minutes) (Lane 6), and A549 treated with Afatinib followed by EGF (0.5 uM for 6 hours, 200ng/ml for 10 minutes) (Lane 7) using GTX25644 EGFR (phospho Tyr1068) antibody.
Dilution : 1:1000

ICC/IF analysis of A431 cells treated with 200 ng/ml of EGF for 10 minutes using GTX25644 EGFR (phospho Tyr1068) antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 for 10 minutes