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EGFR (phospho Tyr1086) antibody

Orthogonal
Anti-EGFR (phospho Tyr1086) antibody used in Western Blot (WB). GTX25650
Anti-EGFR (phospho Tyr1086) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25650
Anti-EGFR (phospho Tyr1086) antibody used in Western Blot (WB). GTX25650
Anti-EGFR (phospho Tyr1086) antibody used in Western Blot (WB). GTX25650

Cat. No. GTX25650

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC

Reactivity

 Human, Mouse
Package
50 μl ($399)
See all EGFR products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
ICC/IF 1:100
IHC Assay dependent
Not tested in other applications.

Calculated MW

134 kDa. ( Note )

Product Note

The full-length EGFR includes the 24 aa signal peptide that is cleaved during EGFR maturation. The Tyr1086 of mature EGFR is equivalent to Tyr1110 of uncleaved EGFR.

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA, 50% Glycerol

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human EGFR that contains tyrosine 1086. The sequence is conserved in mouse and rat.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

epidermal growth factor receptor , ERBB , ERBB1 , HER1 , NISBD2 , PIG61 , mENA

Cellular Localization

Cell membrane,Endoplasmic reticulum membrane,Endosome,Endosome membrane,Nucleus

Background

The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq, Jun 2016]

Database

Research Area

DATA IMAGES

Anti-EGFR (phospho Tyr1086) antibody used in Western Blot (WB). GTX25650

GTX25650 WB Image

Orthogonal

WB analysis of 30 μg of A549 (Lane 1) treated for 10 minutes with 200 ng/ml of EGF (Lane 2), A431 (Lane 3) treated for 10 minutes with 200 ng/ml of EGF (Lane 4) cell lysate using GTX25650 EGFR (phospho Tyr1086) antibody.
Dilution : 1:1000

Anti-EGFR (phospho Tyr1086) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25650

GTX25650 ICC/IF Image

Orthogonal

ICC/IF analysis of A431 cells treated with Afatinib (1uM for 6hrs) followed by EGF (200ng/ml for 10 minutes) using GTX25650 EGFR (phospho Tyr1086) antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 for 15 minutes
Dilution : 1:100 in 0.1% BSA incubated at 4ºC

Anti-EGFR (phospho Tyr1086) antibody used in Western Blot (WB). GTX25650

GTX25650 WB Image

Orthogonal

WB analysis of membrane enriched extracts (30 ug lysate) of A-431 (Lane 1) treated with EGF (200ng/ml for 10 minutes) (Lane 2), A-431 treated with Gefitinib followed by EGF (1uM for 16 hours, 200ng/ml for 10 minutes) (Lane 3), A-431 treated with Afatinib followed by EGF (0.5 uM for 6 hours, 200ng/ml for 10 minutes) (Lane 4), A549 (Lane 5) treated with EGF (200ng/ml for 10 minutes) (Lane 6), and A549 treated with Afatinib followed by EGF (0.5 uM for 6 hours, 200ng/ml for 10 minutes) (Lane 7) using GTX25650 EGFR (phospho Tyr1086) antibody.
Dilution : 1:1000

Anti-EGFR (phospho Tyr1086) antibody used in Western Blot (WB). GTX25650

GTX25650 WB Image

Orthogonal

WB (peptide competition) analysis of A431 cells stimulated with 200 ng/mL EGF for 15 minutes (Lane 2-5) using GTX25650 EGFR (phospho Tyr1086) antibody prior incubated with the non-phosphopeptide corresponding to the phosphopeptide immunogen (Lane 3), a generic phosphotyrosine-containing peptide (Lane 4), or the phosphopeptide immunogen (Lane 5). The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399