APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:1000 |
| 1:100 |
| Assay dependent |
Not tested in other applications.
Calculated MW
Product Note
The full-length EGFR includes the 24 aa signal peptide that is cleaved during EGFR maturation. The Tyr1086 of mature EGFR is equivalent to Tyr1110 of uncleaved EGFR.
PROPERTIES
Form
Liquid
Buffer
PBS pH7.3, 50% Glycerol, 1mg/ml BSA
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human EGFR that contains tyrosine 1086. The sequence is conserved in mouse and rat.
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
epidermal growth factor receptor , ERBB , ERBB1 , HER1 , NISBD2 , PIG61 , mENA
Cellular Localization
Cell membrane,Endoplasmic reticulum membrane,Endosome,Endosome membrane,Nucleus
Background
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq, Jun 2016]
Database
Research Area
DATA IMAGES
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GTX25650 WB Image
WB analysis of 30 μg of A549 (Lane 1) treated for 10 minutes with 200 ng/ml of EGF (Lane 2), A431 (Lane 3) treated for 10 minutes with 200 ng/ml of EGF (Lane 4) cell lysate using GTX25650 EGFR (phospho Tyr1086) antibody.
Dilution : 1:1000
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GTX25650 ICC/IF Image
ICC/IF analysis of A431 cells treated with Afatinib (1uM for 6hrs) followed by EGF (200ng/ml for 10 minutes) using GTX25650 EGFR (phospho Tyr1086) antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 for 15 minutes
Dilution : 1:100 in 0.1% BSA incubated at 4ºC
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GTX25650 WB Image
WB analysis of membrane enriched extracts (30 ug lysate) of A-431 (Lane 1) treated with EGF (200ng/ml for 10 minutes) (Lane 2), A-431 treated with Gefitinib followed by EGF (1uM for 16 hours, 200ng/ml for 10 minutes) (Lane 3), A-431 treated with Afatinib followed by EGF (0.5 uM for 6 hours, 200ng/ml for 10 minutes) (Lane 4), A549 (Lane 5) treated with EGF (200ng/ml for 10 minutes) (Lane 6), and A549 treated with Afatinib followed by EGF (0.5 uM for 6 hours, 200ng/ml for 10 minutes) (Lane 7) using GTX25650 EGFR (phospho Tyr1086) antibody.
Dilution : 1:1000
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GTX25650 WB Image
WB (peptide competition) analysis of A431 cells stimulated with 200 ng/mL EGF for 15 minutes (Lane 2-5) using GTX25650 EGFR (phospho Tyr1086) antibody prior incubated with the non-phosphopeptide corresponding to the phosphopeptide immunogen (Lane 3), a generic phosphotyrosine-containing peptide (Lane 4), or the phosphopeptide immunogen (Lane 5). The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody.
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REFERENCE
There are currently no references for EGFR (phospho Tyr1086) antibody (GTX25650). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for EGFR (phospho Tyr1086) antibody (GTX25650). Be the first to share your experience with this product.
