WB (peptide competition) analysis of A431 cells stimulated with 200 ng/mL EGF (Lane 2-5) for 15 minutes using GTX10643 EGFR (phospho Tyr1148) antibody prior incubated with, non-phosphopeptide corresponding to the phosphopeptide immunogen (Lane 3), a generic phosphotyrosine-containing peptide (Lane 4), or the phosphopeptide immunogen (Lane 5) control. The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody.

ICC/IF analysis of A549 cells using GTX10643 EGFR (phospho Tyr1148) antibody. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.25% Trito X-100 for 10 minutes
Dilution : 1:400 for 30 minutes at room temperature