*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
The full-length EGFR includes the 24 aa signal peptide that is cleaved during EGFR maturation. The Tyr1148 of mature EGFR is equivalent to Tyr1172 of uncleaved EGFR.
PBS pH 7.3, 1mg/ml BSA, 50% glycerol, 0.05% sodium azide
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of EGFR that contains tyrosine 1148. The sequence is conserved in mouse, rat and chicken.
Purified by antigen-affinity chromatography
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Epidermal Growth Factor Receptor,Erbb,Erbb1,Her1,Nisbd2,Pig61,Mena,Egfr
Cell membrane,Endoplasmic reticulum membrane,Endosome,Endosome membrane,Golgi apparatus membrane,Nucleus,Nucleus membrane,Secreted
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq, Jun 2016]
GTX10643 WB Image
WB (peptide competition) analysis of A431 cells stimulated with 200 ng/mL EGF (Lane 2-5) for 15 minutes using GTX10643 EGFR (phospho Tyr1148) antibody prior incubated with, non-phosphopeptide corresponding to the phosphopeptide immunogen (Lane 3), a generic phosphotyrosine-containing peptide (Lane 4), or the phosphopeptide immunogen (Lane 5) control. The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody.
GTX10643 ICC/IF Image
ICC/IF analysis of A549 cells using GTX10643 EGFR (phospho Tyr1148) antibody. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.25% Trito X-100 for 10 minutes
Dilution : 1:400 for 30 minutes at room temperature