APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
A431 (Starvation for 16 hr and then 100 ng/ml EGF treatment for 5 min)
Product Note
The full-length EGFR includes the 24 aa signal peptide that is cleaved during EGFR maturation. The Tyr1148 of mature EGFR is equivalent to Tyr1172 of uncleaved EGFR.
Predict Reactivity
Mouse, Rat, Chicken(>80% identity)
PROPERTIES
Form
Liquid
Buffer
1XPBS (pH7), 1% BSA, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.3 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide surrounding phospho Tyr1148 of human EGFR. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2887036
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
epidermal growth factor receptor , ERBB , ERBB1 , HER1 , NISBD2 , PIG61 , mENA
Cellular Localization
Cell membrane; Single-pass type I membrane protein , Isoform 2: Secreted
Background
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX133594 WB Image
Untreated (–) and treated (+) A431 whole cell extract (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EGFR (phospho Tyr1148) antibody (GTX133594) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX133594 ICC/IF Image
EGFR (phospho Tyr1148) antibody detects EGFR (phospho Tyr1148) protein at cell membrane by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: EGFR (phospho Tyr1148) protein stained by EGFR (phospho Tyr1148) antibody (GTX133594) diluted at 1:500.
Blue: Hoechst 33342 staining.
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REFERENCE
There are currently no references for EGFR (phospho Tyr1148) antibody (GTX133594). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for EGFR (phospho Tyr1148) antibody (GTX133594). Be the first to share your experience with this product.
