*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
A431 (Starvation for 16 hr and then 100 ng/ml EGF treatment for 5 min)
The full-length EGFR includes the 24 aa signal peptide that is cleaved during EGFR maturation. The Tyr1148 of mature EGFR is equivalent to Tyr1172 of uncleaved EGFR.
Mouse, Rat, Chicken(>80% identity)
1XPBS (pH7), 1% BSA, 20% Glycerol
0.025% ProClin 300
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
0.3 mg/ml (Please refer to the vial label for the specific concentration.)
Carrier-protein conjugated synthetic peptide surrounding phospho Tyr1148 of human EGFR. The exact sequence is proprietary.
Purified by antigen-affinity chromatography.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
epidermal growth factor receptor , ERBB , ERBB1 , HER1 , NISBD2 , PIG61 , mENA
Cell membrane; Single-pass type I membrane protein , Isoform 2: Secreted
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq]
GTX133594 WB Image
Untreated (–) and treated (+) A431 whole cell extract (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EGFR (phospho Tyr1148) antibody (GTX133594) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX133594 ICC/IF Image
EGFR (phospho Tyr1148) antibody detects EGFR (phospho Tyr1148) protein at cell membrane by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: EGFR (phospho Tyr1148) protein stained by EGFR (phospho Tyr1148) antibody (GTX133594) diluted at 1:500.
Blue: Hoechst 33342 staining.
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